ArticleMolecular microbiology2010
Transcription, processing and function of CRISPR cassettes in Escherichia coli.
Article in Molecular microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 130 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
130 citing papers in PubMed, 230 citations in OpenAlex.
- Genomic Exploration of CRISPR-Cas Systems in Raoultella species: Diversity and Architecture.Current microbiology · 2026Article
- Gradient metapopulation microfluidic ecologies shape genetic and biofilm drivers of T4r phage resistance in E. coli.NPJ biofilms and microbiomes · 2026Article
- Molecular kinetics dictate population dynamics in CRISPR-based plasmid defense.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- LysR-type regulator LrhA promotes CRISPR-Cas immunity in Escherichia coli.Nucleic acids research · 2026Article
- TheEcoSal Plus · 2025Review
- Article
- Anoxia activates CRISPR-Cas immunity in the mouse intestine.Nature microbiology · 2025Article
- An evolutionary approach to predict the orientation of CRISPR arrays.PLoS computational biology · 2025Article
- Rapid CRISPR-Cas9 target-strand nicking can provide phage resistance by reducing DNA abundance.Nucleic acids research · 2025Article
- The H-NS homologues MvaT and MvaU repress CRISPR-Cas inPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Article
- Cas11 augments Cascade functions in type I-E CRISPR system but is redundant for gene silencing and plasmid interference.The Biochemical journal · 2025Article
- SspA is a transcriptional regulator of CRISPR adaptation in E. coli.Nucleic acids research · 2025Article
- Microfluidic Ecology Unravels the Genetic and Ecological Drivers of T4r Bacteriophage Resistance in E. coli: Insights into Biofilm-Mediated Evolution.Research square · 2024Article
- Comparative Genomics of Clostridioides difficile.Advances in experimental medicine and biology · 2024Article
- Cas1-Cas2 physically and functionally interacts with DnaK to modulate CRISPR Adaptation.Nucleic acids research · 2023Article
- CRISPR-Cas adaptation in Escherichia coli.Bioscience reports · 2023Review
- Molecular Details of DNA Integration by CRISPR-Associated Proteins During Adaptation in Bacteria and Archaea.Advances in experimental medicine and biology · 2023Review
- CRISPR-Cas Controls Cryptic Prophages.International journal of molecular sciences · 2022Article
- Spacer prioritization in CRISPR-Cas9 immunity is enabled by the leader RNA.Nature microbiology · 2022Article
- Type I CRISPR-Cas provides robust immunity but incomplete attenuation of phage-induced cellular stress.Nucleic acids research · 2022Article
70 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
Abstract
CRISPR/Cas, bacterial and archaeal systems of interference with foreign genetic elements such as viruses or plasmids, consist of DNA loci called CRISPR cassettes (a set of variable spacers regularly separated by palindromic repeats) and associated cas genes. When a CRISPR spacer sequence exactly matches a sequence in a viral genome, the cell can become resistant to the virus. The CRISPR/Cas systems function through small RNAs originating from longer CRISPR cassette transcripts. While laboratory strains of Escherichia coli contain a functional CRISPR/Cas system (as judged by appearance of phage resistance at conditions of artificial co-overexpression of Cas genes and a CRISPR cassette engineered to target a λ-phage), no natural phage resistance due to CRISPR system function was observed in this best-studied organism and no E. coli CRISPR spacer matches sequences of well-studied E. coli phages. To better understand the apparently 'silent'E. coli CRISPR/Cas system, we systematically characterized processed transcripts from CRISPR cassettes. Using an engineered strain with genomically located spacer matching phage λ we show that endogenous levels of CRISPR cassette and cas genes expression allow only weak protection against infection with the phage. However, derepression of the CRISPR/Cas system by disruption of the hns gene leads to high level of protection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.