ArticleStem cells and development2011
High-throughput tracking of pluripotent human embryonic stem cells with dual fluorescence resonance energy transfer molecular beacons.
Article in Stem cells and development, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 40 citations in OpenAlex.
- Embryonic Stem Cells in Clinical Trials: Current Overview of Developments and Challenges.Advances in experimental medicine and biology · 2021Review
- Nucleic-Acid Structures as Intracellular Probes for Live Cells.Advanced materials (Deerfield Beach, Fla.) · 2020Review
- Preparation of cationized gelatin nanospheres incorporating molecular beacon to visualize cell apoptosis.Scientific reports · 2018Article
- Technical approaches to induce selective cell death of pluripotent stem cells.Cellular and molecular life sciences : CMLS · 2017Review
- Single cell biology beyond the era of antibodies: relevance, challenges, and promises in biomedical research.Cellular and molecular life sciences : CMLS · 2017Review
- Single-cell differences in matrix gene expression do not predict matrix deposition.Nature communications · 2016Article
- Development of a universal RNA beacon for exogenous gene detection.Stem cells translational medicine · 2015Article
- Current methods and challenges in the comprehensive characterization of human pluripotent stem cells.Stem cell reviews and reports · 2015Review
- Gene expression-based enrichment of live cells from adipose tissue produces subpopulations with improved osteogenic potential.Stem cell research & therapy · 2014Article
- Molecular beacon-enabled purification of living cells by targeting cell type-specific mRNAs.Nature protocols · 2014Article
- Live-cell, temporal gene expression analysis of osteogenic differentiation in adipose-derived stem cells.Tissue engineering. Part A · 2014Article
- High expression of hTERT and stemness genes in BORIS/CTCFL positive cells isolated from embryonic cancer cells.PloS one · 2014Article
- Purification of cardiomyocytes from differentiating pluripotent stem cells using molecular beacons that target cardiomyocyte-specific mRNA.Circulation · 2013Article
- Live-cell, temporal gene expression analysis of osteogenic differentiation in adipose-derived stem cells.Tissue engineering. Part A · 2013Article
- Strategies for enrichment and selection of stem cell-derived tissue precursors.Stem cell research & therapy · 2012Review
- Developmental effects of tobacco smoke exposure during human embryonic stem cell differentiation are mediated through the transforming growth factor-β superfamily member, Nodal.Differentiation; research in biological diversity · 2012Article
- Myocardial improvement with human embryonic stem cell-derived cardiomyocytes enriched by p38MAPK inhibition.Cytotherapy · 2012Article
- Sorting live stem cells based on Sox2 mRNA expression.PloS one · 2012Article
- Review
- Stem cell-based strategies for the treatment of type 1 diabetes mellitus.Expert opinion on biological therapy · 2011Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Pluripotent human embryonic stem cells (hESCs) provide an unprecedented opportunity for the study of human tissue development, and the development of cell-based therapies for human disease. To realize these potential advances, however, methods for monitoring expression of intracellular proteins in live hESCs without altering cellular properties are needed. Molecular beacons are single-stranded oligonucleotides that have been employed to assay gene expression. To test their potential for high-throughput isolation of hESCs, we developed a dual fluorescence resonance energy transfer (FRET) molecular beacon system using fluorescence-activated cell sorting (FACS) with Oct4 as a target. We demonstrate that Oct4 can be detected by FRET using confocal microscopy, that this can be applied in a high-throughput manner to the identification and isolation of Oct4-expressing hESCs by FACS, that FRET-positive hESCs demonstrate pluripotency in culture and in vivo, and that hESCs transfected with molecular beacons demonstrate normal growth rates and oligonucleotide extinction over time. These studies demonstrate that FRET-based FACS using molecular beacons provides a useful tool for isolating Oct4-expressing pluripotent hESCs, and may also be adapted to selecting differentiating hESCs at specific developmental time points determined by transcription factor expression without functional or genomic alteration. As such, it provides an important new method for high-throughput isolation of hESC-derived tissue-specific precursors for analytic and therapeutic purposes.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.