ArticleCytoskeleton (Hoboken, N.J.)2010
Modeling capping protein FRAP and CALI experiments reveals in vivo regulation of actin dynamics.
Article in Cytoskeleton (Hoboken, N.J.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 20 citations in OpenAlex.
- Celebrating the creative scientific life of Ken Jacobson.Biophysical journal · 2023Article
- Beyond analytic solution: Analysis of FRAP experiments by spatial simulation of the forward problem.Biophysical journal · 2023Article
- Following the footprints of variability during filopodial growth.European biophysics journal : EBJ · 2020Article
- Simultaneous quantification of actin monomer and filament dynamics with modeling-assisted analysis of photoactivation.Journal of cell science · 2016Article
- Model of turnover kinetics in the lamellipodium: implications of slow- and fast- diffusing capping protein and Arp2/3 complex.Physical biology · 2016Article
- There is more than one way to model an elephant. Experiment-driven modeling of the actin cytoskeleton.Biophysical journal · 2013Review
- Distributed actin turnover in the lamellipodium and FRAP kinetics.Biophysical journal · 2013Article
- Stoichiometry of Nck-dependent actin polymerization in living cells.The Journal of cell biology · 2012Article
- Advanced fluorescence microscopy techniques--FRAP, FLIP, FLAP, FRET and FLIM.Molecules (Basel, Switzerland) · 2012Review
- Growth cone travel in space and time: the cellular ensemble of cytoskeleton, adhesion, and membrane.Neuron · 2012Review
- Assessment of cellular mechanisms contributing to cAMP compartmentalization in pulmonary microvascular endothelial cells.American journal of physiology. Cell physiology · 2012Article
- Spatial modeling of cell signaling networks.Methods in cell biology · 2012Article
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
Abstract
To gain insights on cellular mechanisms regulating actin polymerization, we used the Virtual Cell to model fluorescence recovery after photobleaching (FRAP) and chromophore-assisted laser inactivation (CALI) experiments on EGFP-capping protein (EGFP-CP). Modeling the FRAP kinetics demonstrated that the in vivo rate for the dissociation of CP from actin filaments is much faster (approximately 0.1 s(-1)) than that measured in vitro (0.01-0.0004 s(-1)). The CALI simulation revealed that in order to induce sustainable changes in cell morphology after CP inactivation, the cells should exhibit anticapping ability. We included the VASP protein as the anticapping agent in the modeling scheme. The model predicts that VASP affinity for barbed ends has a cooperative dependence on the concentration of VASP-barbed end complexes. This dependence produces a positive feedback that stabilizes the complexes and allows sustained growth at clustered filament tips. We analyzed the range of laser intensities that are sufficient to induce changes in cell morphology. This analysis demonstrates that FRAP experiments with EGFP-CP can be performed safely without changes in cell morphology, because, the intensity of the photobleaching beam is not high enough to produce the critical concentration of free barbed ends that will induce filament growth before diffusional replacement of EGFP-CP occurs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.