ArticleNMR in biomedicine2010
Choline kinase overexpression increases invasiveness and drug resistance of human breast cancer cells.
Article in NMR in biomedicine, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 64 citations in OpenAlex.
- Prognostic value of metabolic response in breast cancer patients receiving neoadjuvant chemotherapy.BMC cancer · 2012Trial
- Multidimensional pH-Temperature Mapping of SABRE-SHEATHThe journal of physical chemistry. B · 2025Article
- Review
- The Utility of [18]F-Fluorocholine Positron Emission Computed Tomography and [18]F-Fluorodeoxyglucose Positron Emission Tomography-Computed Tomography in Evaluating Breast Cancer Phenotypes: A Pilot Study.The Eurasian journal of medicine · 2024Article
- Phosphatidylcholine-Specific Phospholipase C as a Promising Drug Target.Molecules (Basel, Switzerland) · 2023Review
- The 'omics of obesity in B-cell acute lymphoblastic leukemia.Journal of the National Cancer Institute. Monographs · 2023Article
- Metabolic changes in glioblastomas in response to choline kinase inhibition: In vivo MRS in rodent models.NMR in biomedicine · 2023Article
- Bilayer Forming Phospholipids as Targets for Cancer Therapy.International journal of molecular sciences · 2022Review
- Phosphatidylcholine-Derived Lipid Mediators: The Crosstalk Between Cancer Cells and Immune Cells.Frontiers in immunology · 2022Review
- Choline Kinase: An Unexpected Journey for a Precision Medicine Strategy in Human Diseases.Pharmaceutics · 2021Review
- Microenvironmental Factors Modulating Tumor Lipid Metabolism: Paving the Way to Better Antitumoral Therapy.Frontiers in oncology · 2021Review
- TGFβ-induced metabolic reprogramming during epithelial-to-mesenchymal transition in cancer.Cellular and molecular life sciences : CMLS · 2020Review
- MRI and MRS of intact perfused cancer cell metabolism, invasion, and stromal cell interactions.NMR in biomedicine · 2019Review
- Proton MR spectroscopy in the breast: Technical innovations and clinical applications.Journal of magnetic resonance imaging : JMRI · 2019Review
- Choline kinase inhibitors EB-3D and EB-3P interferes with lipid homeostasis in HepG2 cells.Scientific reports · 2019Article
- Choline Kinase Emerges as a Promising Drug Target in Gram-Positive Bacteria.Frontiers in microbiology · 2019Article
- Molecular causes of elevated phosphoethanolamine in breast and pancreatic cancer cells.NMR in biomedicine · 2018Article
- Parallel Colorimetric Quantification of Choline and Phosphocholine as a Method for Studying Choline Kinase Activity in Complex Mixtures.Antibiotics (Basel, Switzerland) · 2018Article
- Recent Advances in Comprehending the Signaling Pathways Involved in the Progression of Breast Cancer.International journal of molecular sciences · 2017Review
- Applications of high-resolution magic angle spinning MRS in biomedical studies II-Human diseases.NMR in biomedicine · 2017Review
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
Abstract
A direct correlation exists between increased choline kinase (Chk) expression, and the resulting increase of phosphocholine levels, and histological tumor grade. To better understand the function of Chk and choline phospholipid metabolism in breast cancer we have stably overexpressed one of the two isoforms of Chk-alpha known to be upregulated in malignant cells, in non-invasive MCF-7 human breast cancer cells. Dynamic tracking of cell invasion and cell metabolism were studied with a magnetic resonance (MR) compatible cell perfusion assay. The MR based invasion assay demonstrated that MCF-7 cells overexpressing Chk-alpha (MCF-7-Chk) exhibited an increase of invasion relative to control MCF-7 cells (0.84 vs 0.3). Proton MR spectroscopy studies showed significantly higher phosphocholine and elevated triglyceride signals in Chk overexpressing clones compared to control cells. A test of drug resistance in MCF-7-Chk cells revealed that these cells had an increased resistance to 5-fluorouracil and higher expression of thymidylate synthase compared to control MCF-7 cells. To further characterize increased drug resistance in these cells, we performed rhodamine-123 efflux studies to evaluate drug efflux pumps. MCF-7-Chk cells effluxed twice as much rhodamine-123 compared to MCF-7 cells. Chk-alpha overexpression resulted in MCF-7 human breast cancer cells acquiring an increasingly aggressive phenotype, supporting the role of Chk-alpha in mediating invasion and drug resistance, and the use of phosphocholine as a biomarker of aggressive breast cancers.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.