ArticleThe Journal of membrane biology2010
Gene transfer: how can the biological barriers be overcome?
Article in The Journal of membrane biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 71 citations in OpenAlex.
- Anticodon-edited transfer RNAs (ACE-tRNAs) encoded as therapeutic nonviral minimal DNA vectors.Nucleic acids research · 2026Article
- Smart micro/nanoneedles for gene delivery.Iranian journal of basic medical sciences · 2026Review
- Anticodon Edited Transfer RNAs (ACE-tRNAs) Encoded as Therapeutic Nonviral Minimal DNA Vectors.bioRxiv : the preprint server for biology · 2025Article
- Single-Cell Electroporation with Real-Time Impedance Assessment Using a Constriction Microchannel.Micromachines · 2020Article
- Niosome-Based Approach for In Situ Gene Delivery to Retina and Brain Cortex as Immune-Privileged Tissues.Pharmaceutics · 2020Review
- Enhanced Delivery of Plasmid DNA to Skeletal Muscle Cells using a DLC8-Binding Peptide and ASSLNIA-Modified PAMAM Dendrimer.Molecular pharmaceutics · 2019Article
- Translation, but not transfection limits clinically relevant, exogenous mRNA based induction of alpha-4 integrin expression on human mesenchymal stem cells.Scientific reports · 2017Article
- Modified mRNA as a therapeutic tool to induce cardiac regeneration in ischemic heart disease.Wiley interdisciplinary reviews. Systems biology and medicine · 2017Review
- Efficient In Vitro Electropermeabilization of Reconstructed Human Dermal Tissue.The Journal of membrane biology · 2015Article
- A double-pulse approach for electrotransfection.The Journal of membrane biology · 2014Article
- Quantitative evaluation of DNA dissociation from liposome carriers and DNA escape from endosomes during lipid-mediated gene delivery.Human gene therapy methods · 2014Article
- Single-site sonoporation disrupts actin cytoskeleton organization.Journal of the Royal Society, Interface · 2014Article
- Gene Therapy Used in Cancer Treatment.Biomedicines · 2014Review
- Mechanisms of microbubble-facilitated sonoporation for drug and gene delivery.Therapeutic delivery · 2014Review
- Physical methods for intracellular delivery: practical aspects from laboratory use to industrial-scale processing.Journal of laboratory automation · 2014Review
- Gene therapy delivery systems for enhancing viral and nonviral vectors for cardiac diseases: current concepts and future applications.Human gene therapy · 2013Review
- Nanosecond electric pulse effects on gene expression.The Journal of membrane biology · 2013Article
- Microfluidic electroporation for cellular analysis and delivery.Lab on a chip · 2013Review
- Improving ultrasound gene transfection efficiency by controlling ultrasound excitation of microbubbles.Journal of controlled release : official journal of the Controlled Release Society · 2013Article
- Myocardial gene transfer: routes and devices for regulation of transgene expression by modulation of cellular permeability.Human gene therapy · 2013Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Physical methods represent a promising approach for the safe delivery of therapeutic plasmid DNA in genetic and acquired human diseases. However, their development in clinics is limited by their low efficacy. At the cellular level, efficient gene transfer is dependent on several factors including extracellular matrix, plasmid DNA uptake and nucleocytoplasmic transport. We review the main barriers that plasmid DNA encounters from the extracellular environment toward the interior of the cell and the different strategies developed to overcome these biological barriers. Diffusional and metabolic fences of the extracellular matrix and the cytoplasm affect plasmid DNA uptake. These barriers reduce the number of intact plasmids that reach the nucleus. Nuclear uptake of plasmid DNA further requires either an increase of nuclear permeability or an active nuclear transport via the nuclear pore. A better understanding of the cellular and molecular bases of the physical gene-transfer process may provide strategies to overcome those obstacles that highly limit the efficiency and use of gene-delivery methods.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.