ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2010
iPBS: a universal method for DNA fingerprinting and retrotransposon isolation.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
77 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Traditional System Versus DNA Barcoding in Identification of Bamboo Species: A Systematic Review.Molecular biotechnology · 2021Pooled it
- Progresses of transposon research inSynthetic and systems biotechnology · 2026Review
- Advances in molecular breeding of medicinal plants.Molecular horticulture · 2026Review
- Exploring Genetic Diversity in a Core Collection of Aegilops tauschii Coss. Populations Using iPBS and SCoT Markers.Biochemical genetics · 2026Article
- Genetic diversity and population structure of Passiflora spp. using inter-primer binding site (iPBS) - retrotransposon markers.Molecular biology reports · 2026Article
- Stones in genomic storms: transposable element-driven variation in Prunus fruit trees.Mobile DNA · 2026Review
- Integrated Analysis of Genetic, Spectral, Phenotypic, and Stress-Resistant Traits inPlants (Basel, Switzerland) · 2026Article
- Host Factors Promoting the LTR Retrotransposon Life Cycle in Plant Cells: Current Knowledge and Future Directions.International journal of molecular sciences · 2025Review
- Enhancing and comparison of yield components through diallel analysis in FScientific reports · 2025Article
- Genetic Diversity ofGenes · 2025Article
- Enhanced genetic diversity assessment of Deschampsia Antarctica populations from maritime Antarctica using inter-primer binding site (iPBS) retrotransposon markers.Molecular biology reports · 2025Article
- Development of Molecular Tools to Identify the Avocado (International journal of molecular sciences · 2025Article
- Revealing the genetic diversity and population structure in lentil (Lens culinaris) germplasm using inter-primer binding site (iPBS)-retrotransposon markers.BMC plant biology · 2025Article
- Exploring Genetic Diversity and Inter-/Intraspecific Polymorphism inInternational journal of molecular sciences · 2025Article
- Drought-Induced genomic and epigenetic variations in Quinoa genotypes revealed by iPBS and CRED-iPBS marker systems.Scientific reports · 2025Article
- Powdery mildew resistance prediction in Barley (Hordeum Vulgare L) with emphasis on machine learning approaches.Scientific reports · 2025Article
- The Combination of Start-Codon-Targeted (SCoT) andInternational journal of molecular sciences · 2025Article
- Integrating in situ strategies and molecular genetics for the conservation of the endangered Azorean endemic plant Lotus azoricus.Scientific reports · 2025Article
- Conservation genetic evaluation of Juniperus communis sensu lato in Slovakia.Scientific reports · 2025Article
- Metabolomic adaptations and genetic polymorphism in ecopopulations ofFrontiers in plant science · 2025Article
17 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Molecular markers are essential in plant and animal breeding and biodiversity applications, in human forensics, and for map-based cloning of genes. The long terminal repeat (LTR) retrotransposons are well suited as molecular markers. As dispersed and ubiquitous transposable elements, their "copy and paste" life cycle of replicative transposition leads to new genome insertions without excision of the original element. Both the overall structure of retrotransposons and the domains responsible for the various phases of their replication are highly conserved in all eukaryotes. Nevertheless, up to a year has been required to develop a retrotransposon marker system in a new species, involving cloning and sequencing steps as well as the development of custom primers. Here, we describe a novel PCR-based method useful both as a marker system in its own right and for the rapid isolation of retrotransposon termini and full-length elements, making it ideal for "orphan crops" and other species with underdeveloped marker systems. The method, iPBS amplification, is based on the virtually universal presence of a tRNA complement as a reverse transcriptase primer binding site (PBS) in LTR retrotransposons. The method differs from earlier retrotransposon isolation methods because it is applicable not only to endogenous retroviruses and retroviruses, but also to both Gypsy and Copia LTR retrotransposons, as well as to non-autonomous LARD and TRIM elements, throughout the plant kingdom and to animals. Furthermore, the inter-PBS amplification technique as such has proved to be a powerful DNA fingerprinting technology without the need for prior sequence knowledge.
Indexed as
Identifiers
20623102What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.