ArticleLab on a chip2010
Single-molecule imaging of NGF axonal transport in microfluidic devices.
Article in Lab on a chip, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
39 citing papers in PubMed, 89 citations in OpenAlex.
- Nanomagnetic Guidance Shapes the Structure-Function Relationship of Developing Cortical Networks.Nano letters · 2024Article
- Neural Tracing Protein-Functionalized Nanoparticles Capable of Fast Retrograde Axonal Transport in Live Neurons.Small (Weinheim an der Bergstrasse, Germany) · 2024Article
- Integrated In-Plane Nanofluidic Devices for Resistive-Pulse Sensing.Annual review of analytical chemistry (Palo Alto, Calif.) · 2024Review
- Microfluidics for Neuronal Cell and Circuit Engineering.Chemical reviews · 2022Review
- Calcineurin and huntingtin form a calcium-sensing machinery that directs neurotrophic signals to the nucleus.Science advances · 2022Article
- Microfluidic platforms for single neuron analysis.Materials today. Bio · 2022Review
- Molecular-Scale Dynamics of Long Range Retrograde Brain-Derived Neurotrophic Factor Transport Shaped by Cellular Spatial Context.Frontiers in neuroscience · 2022Article
- Live Imaging of Axonal Transport in the Adult Drosophila Central Nervous System.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Multimodal single-molecule microscopy with continuously controlled spectral resolution.Biophysical reports · 2021Article
- Surface plasmon-coupled emission imaging for biological applications.Analytical and bioanalytical chemistry · 2020Review
- Growth and elongation of axons through mechanical tension mediated by fluorescent-magnetic bifunctional FeJournal of nanobiotechnology · 2020Article
- On-Demand Intracellular Delivery of Single Particles in Single Cells by 3D Hollow Nanoelectrodes.Nano letters · 2019Article
- Raftophilic rhodopsin-clusters offer stochastic platforms for G protein signalling in retinal discs.Communications biology · 2019Article
- Dynamic Clustering of Dyneins on Axonal Endosomes: Evidence from High-Speed Darkfield Imaging.Biophysical journal · 2018Article
- Swedish Nerve Growth Factor Mutation (NGFThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- Rotation of endosomes demonstrates coordination of molecular motors during axonal transport.Science advances · 2018Article
- Modeling the neuron as a nanocommunication system to identify spatiotemporal molecular events in neurodegenerative disease.International journal of nanomedicine · 2018Article
- Visualizing endocytic recycling and trafficking in live neurons by subdiffractional tracking of internalized molecules.Nature protocols · 2017Article
- A new method for quantifying mitochondrial axonal transport.Protein & cell · 2016Article
- In vivo imaging of axonal transport in murine motor and sensory neurons.Journal of neuroscience methods · 2016Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Nerve growth factor (NGF) signaling begins at the nerve terminal, where it binds and activates membrane receptors and subsequently carries the cell-survival signal to the cell body through the axon. A recent study revealed that the majority of endosomes contain a single NGF molecule, which makes single-molecule imaging an essential tool for NGF studies. Despite being an increasingly popular technique, single-molecule imaging in live cells is often limited by background fluorescence. Here, we employed a microfluidic culture platform to achieve background reduction for single-molecule imaging in live neurons. Microfluidic devices guide the growth of neurons and allow separately controlled microenvironment for cell bodies or axon termini. Designs of microfluidic devices were optimized and a three-compartment device successfully achieved direct observation of axonal transport of single NGF when quantum dot labeled NGF (Qdot-NGF) was applied only to the distal-axon compartment while imaging was carried out exclusively in the cell-body compartment. Qdot-NGF was shown to move exclusively toward the cell body with a characteristic stop-and-go pattern of movements. Measurements at various temperatures show that the rate of NGF retrograde transport decreased exponentially over the range of 36-14 degrees C. A 10 degrees C decrease in temperature resulted in a threefold decrease in the rate of NGF retrograde transport. Our successful measurements of NGF transport suggest that the microfluidic device can serve as a unique platform for single-molecule imaging of molecular processes in neurons.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.