Evidence map›Paper›PMID 20622988›Full record

ArticleNano biomedicine and engineering2010

Rapid Whole Blood Bioassays using Microwave-Accelerated Metal-Enhanced Fluorescence.

Kadir Aslan

Open access · diamondAbstract read
In one paragraph

Article in Nano biomedicine and engineering, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
1.9field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 22 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

1 author at 1 institution in 1 country.

Kadir AslanMorgan State University, Department of Chemistry, 1700 East Cold Spring Lane Baltimore, MD 21251.
Morgan State University · US

Funding

Ultra Fast and Sensitive Fluorescence-Based Clinical AssaysK25EB007565 · NIBIB · UNIVERSITY OF MD BIOTECHNOLOGY INSTITUTE · PI ASLAN, KADIR · 2008 to 2011
$607k
NIBIB NIH HHS K25 EB007565
6 · The paper itself

Abstract

The proof-of-principle demonstration of rapid whole blood bioassays based on microwave-accelerated metal-enhanced fluorescence (MAMEF) method using silver nanoparticle-deposited surfaces is presented. In this regard, spherical silver nanoparticles were deposited onto glass slides (silver nanoparticle films, SNFs) in a highly reproducible manner, which was assessed by optical absorption spectroscopy. Atomic force microscopy was employed to determine the size of the deposited silver nanoparticles. A model bioassay, based on the well-known interactions of biotinylated bovine serum albumin (b-BSA) and streptavidin was constructed on SNFs. The model bioassay was run at room temperature (metal-enhanced fluorescence (MEF)-based bioassay without microwave heating) for 60 minutes and with microwave heating (MAMEF-based bioassay) for 1 minute. In contrast to MEF-based bioassays that only allowed the use of samples in buffer solution, MAMEF-based bioassays afforded the use of whole blood samples. A lower detection limit of 1 nM and 0.01 nM for b-BSA was determined in MEF-based and MAMEF-based bioassays, respectively.

Identifiers

PMID20622988
PMCPMC2901134
OpenAlexW2157389220

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.