ArticleNature nanotechnology2010
Rationally designed logic integration of regulatory signals in mammalian cells.
Article in Nature nanotechnology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 106 citations in OpenAlex.
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- The Blueprint of Logical Decisions in a NF-κB Signaling System.ACS omega · 2024Article
- Hybrid Boolean gates show that Cas12c controls transcription activation effectively in the yeastFrontiers in bioengineering and biotechnology · 2023Article
- Synthetic gene circuits for cell state detection and protein tuning in human pluripotent stem cells.Molecular systems biology · 2022Article
- Genetic physical unclonable functions in human cells.Science advances · 2022Article
- Gene-circuit therapy on the horizon: synthetic biology tools for engineered therapeutics.Acta biochimica Polonica · 2021Review
- A synthetic circuit for buffering gene dosage variation between individual mammalian cells.Nature communications · 2021Article
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- Synthetic Biology Based on Genetic Logic Circuit, Using the Expression of Drug Resistance, BCRP Pump in MCF-7 Cancer Cell Line.Iranian journal of pharmaceutical research : IJPR · 2020Article
- Synthetic control systems for high performance gene expression in mammalian cells.Nucleic acids research · 2018Article
- Designing cell function: assembly of synthetic gene circuits for cell biology applications.Nature reviews. Molecular cell biology · 2018Review
- Mapping the operational landscape of microRNAs in synthetic gene circuits.NPJ systems biology and applications · 2018Article
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- Model-guided combinatorial optimization of complex synthetic gene networks.Molecular systems biology · 2016Article
- Synthetic Biology Platform for Sensing and Integrating Endogenous Transcriptional Inputs in Mammalian Cells.Cell reports · 2016Article
- Engineering Gene Circuits for Mammalian Cell-Based Applications.Cold Spring Harbor perspectives in biology · 2016Review
- RNA-based gene circuits for cell regulation.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2016Review
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5 authors at 3 institutions in 1 country.
Funding
Abstract
Molecular-level information processing is essential for 'smart' in vivo nanosystems. Natural molecular computing, such as the regulation of messenger RNA (mRNA) synthesis by special proteins called transcription factors, has inspired engineered systems that can control the levels of mRNA with certain combinations of transcription factors. Here, we show an alternative approach to achieving general-purpose control of mRNA and protein levels by logic integration of transcription factor input signals in mammalian cells. The transcription factors regulate synthetic genes coding for small regulatory RNAs (called microRNAs), which, in turn, control the mRNA of interest (the output) via an RNA interference pathway. The simplicity of these modular interactions makes it possible, in theory, to implement any arbitrary logic relation between the transcription factors and the output. We construct, test and optimize increasingly complex circuits with up to three transcription factor inputs, establishing a platform for in vivo molecular computing.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.