ArticleApplied and environmental microbiology2010
Quantification of Shiga toxin-converting bacteriophages in wastewater and in fecal samples by real-time quantitative PCR.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 63 citations in OpenAlex.
- Article
- Genomic and functional characterization ofFrontiers in microbiology · 2026Article
- An Appraisal of Bacteriophage Isolation Techniques from Environment.Microbial ecology · 2022Review
- Bacteriophages in sewage: abundance, roles, and applications.FEMS microbes · 2022Article
- Characteristics of Bacteriophage Isolates and Expression of Shiga Toxin Genes Transferred to Non Shiga Toxin-ProducingJournal of microbiology and biotechnology · 2021Article
- Bacteriophages in Biological Wastewater Treatment Systems: Occurrence, Characterization, and Function.Frontiers in microbiology · 2021Review
- Persistence of a Stx-Encoding Bacteriophage in Minced Meat Investigated by Application of an Improved DNA Extraction Method and Digital Droplet PCR.Frontiers in microbiology · 2020Article
- Article
- Discovery of Shiga Toxin-ProducingFrontiers in microbiology · 2019Article
- Article
- Induction of Shiga Toxin-Encoding Prophage by Abiotic Environmental Stress in Food.Applied and environmental microbiology · 2017Article
- Contribution of cropland to the spread of Shiga toxin phages and the emergence of new Shiga toxin-producing strains.Scientific reports · 2017Article
- Real-Time qPCR as a Method for Detection of Antibody-Neutralized Phage Particles.Frontiers in microbiology · 2017Article
- The degradation of two fluoroquinolone based antimicrobials by SilA, an alkaline laccase from Streptomyces ipomoeae.World journal of microbiology & biotechnology · 2016Article
- An Environmental Shiga Toxin-Producing Escherichia coli O145 Clonal Population Exhibits High-Level Phenotypic Variation That Includes Virulence Traits.Applied and environmental microbiology · 2016Article
- UV-Sensitivity of Shiga Toxin-Converting Bacteriophage Virions Φ24B, 933W, P22, P27 and P32.Toxins · 2015Article
- Role of Verocytotoxigenic Escherichia Coli in the Swine Production Chain.Italian journal of food safety · 2015Review
- Improving detection of Shiga toxin-producing Escherichia coli by molecular methods by reducing the interference of free Shiga toxin-encoding bacteriophages.Applied and environmental microbiology · 2015Article
- Whole genome sequence comparison of vtx2-converting phages from Enteroaggregative Haemorrhagic Escherichia coli strains.BMC genomics · 2014Article
- Capture and detection of T7 bacteriophages on a nanostructured interface.ACS applied materials & interfaces · 2014Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Shiga toxin-converting bacteriophages (Stx phages) are involved in the pathogenicity of some enteric bacteria, such as Escherichia coli O157:H7. Stx phages are released from their bacterial hosts after lytic induction and remain free in the environment. Samples were analyzed for the presence of free Stx phages by an experimental approach based on the use of real-time quantitative PCR (qPCR), which enables stx to be detected in the DNA from the viral fraction of each sample. A total of 150 samples, including urban raw sewage samples, wastewater samples with fecal contamination from cattle, pigs, and poultry, and fecal samples from humans and diverse animals, were used in this study. Stx phages were detected in 70.0% of urban sewage samples (10 to 10(3) gene copies [GC] per ml) and in 94.0% of animal wastewater samples of several origins (10 to 10(10) GC per ml). Eighty-nine percent of cattle fecal samples were positive for Stx phages (10 to 10(5) GC per g of sample), as were 31.8% of other fecal samples of various origins (10 to 10(4) GC per g of sample). The stx(2) genes and stx(2) variants were detected in the viral fraction of some of the samples after sequencing of stx(2) fragments amplified by conventional PCR. The occurrence and abundance of Stx phages in the extraintestinal environment confirm the role of Stx phages as a reservoir of stx in the environment.
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