ArticleApplied and environmental microbiology2010
Extraction of mRNA from soil.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed, 1 synthesis or guideline pooled it, 116 citations in OpenAlex.
- Stool metatranscriptomics: A technical guideline for mRNA stabilisation and isolation.BMC genomics · 2015Guideline
- Elevated Atmospheric CoCurrent microbiology · 2024Article
- Evaluation of commercial RNA extraction kits for long-read metatranscriptomics in soil.Microbial genomics · 2024Article
- Role of methanotrophic communities in atmospheric methane oxidation in paddy soils.Frontiers in microbiology · 2024Article
- Clearing the air: unraveling past and guiding future research in atmospheric chemosynthesis.Microbiology and molecular biology reviews : MMBR · 2023Review
- Elevated atmospheric COBMC microbiology · 2023Article
- Microbial-Based Products to Control Soil-Borne Pathogens: Methods to Improve Efficacy and to Assess Impacts on Microbiome.Microorganisms · 2023Review
- Elevated Atmospheric COMicrobial ecology · 2022Article
- Soil metatranscriptome demonstrates a shift in C, N, and S metabolisms of a grassland ecosystem in response to elevated atmospheric COFrontiers in microbiology · 2022Article
- Evaluation of the Respiratory Microbiome and the Use of Tracheal Lavage as a Diagnostic Tool in Kemp's Ridley Sea Turtles (Animals : an open access journal from MDPI · 2021Article
- Rootstocks Shape Their Microbiome-Bacterial Communities in the Rhizosphere of Different Grapevine Rootstocks.Microorganisms · 2021Article
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- Enteromorpha prolifera Diet Drives Intestinal Microbiome Composition in Siganus oramin.Current microbiology · 2021Article
- Article
- Active Soil Nitrifying Communities Revealed byApplied and environmental microbiology · 2020Article
- Elucidating the impact of microbial community biodiversity on pharmaceutical biotransformation during wastewater treatment.Microbial biotechnology · 2018Article
- Metatranscriptomics reveals a differential temperature effect on the structural and functional organization of the anaerobic food web in rice field soil.Microbiome · 2018Article
- Microbial effects of part-stream low-frequency ultrasonic pretreatment on sludge anaerobic digestion as revealed by high-throughput sequencing-based metagenomics and metatranscriptomics.Biotechnology for biofuels · 2018Article
- Lineage overwhelms environmental conditions in determining rhizosphere bacterial community structure in a cosmopolitan invasive plant.Nature communications · 2017Article
- Metatranscriptomic Evidence for Direct Interspecies Electron Transfer between Geobacter and Methanothrix Species in Methanogenic Rice Paddy Soils.Applied and environmental microbiology · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here, we report an efficient method for extracting high-quality mRNA from soil. Key steps in the isolation of total RNA were low-pH extraction (pH 5.0) and Q-Sepharose chromatography. The removal efficiency of humic acids was 94 to 98% for all soils tested. To enrich mRNA, subtractive hybridization of rRNA was most efficient. Subtractive hybridization may be followed by exonuclease treatment if the focus is on the analysis of unprocessed mRNA. The total extraction method can be completed within 8 h, resulting in enriched mRNA ranging from 200 bp to 4 kb in size.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.