ArticleAmerican journal of physiology. Heart and circulatory physiology2010
In vivo assessment of artery smooth muscle [Ca2+]i and MLCK activation in FRET-based biosensor mice.
Article in American journal of physiology. Heart and circulatory physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 25 citations in OpenAlex.
- NO-induced vasodilation correlates directly with BP in smooth muscle-Na/Ca exchanger-1-engineered mice: elevated BP does not attenuate endothelial function.American journal of physiology. Heart and circulatory physiology · 2021Article
- NaAmerican journal of physiology. Heart and circulatory physiology · 2019Article
- Imaging sympathetic neurogenic CaAutonomic neuroscience : basic & clinical · 2017Review
- Pivotal role of α2 NaThe Journal of physiology · 2016Review
- PC-PLC/sphingomyelin synthase activity plays a central role in the development of myogenic tone in murine resistance arteries.American journal of physiology. Heart and circulatory physiology · 2015Article
- A method for noninvasive longitudinal measurements of [Ca2+] in arterioles of hypertensive optical biosensor mice.American journal of physiology. Heart and circulatory physiology · 2014Article
- Correlative intravital imaging of cGMP signals and vasodilation in mice.Frontiers in physiology · 2014Article
- Ca(2+) signaling in arterioles and small arteries of conscious, restrained, optical biosensor mice.Frontiers in physiology · 2014Article
- Should we be sympathetic to angiotensin II infusion?The Journal of physiology · 2013Article
- Intravital Förster resonance energy transfer imaging reveals elevated [Ca2+]i and enhanced sympathetic tone in femoral arteries of angiotensin II-infused hypertensive biosensor mice.The Journal of physiology · 2013Article
- Vascular tone and Ca(2+) signaling in murine cremaster muscle arterioles in vivo.Microcirculation (New York, N.Y. : 1994) · 2013Article
- Application of FRET probes in the analysis of neuronal plasticity.Frontiers in neural circuits · 2013Review
- Article
- How NaCl raises blood pressure: a new paradigm for the pathogenesis of salt-dependent hypertension.American journal of physiology. Heart and circulatory physiology · 2012Review
- Real-time intravital imaging of cancer models.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2011Review
- Intravital macrozoom imaging and automated analysis of endothelial cell calcium signals coincident with arteriolar dilation in Cx40(BAC) -GCaMP2 transgenic mice.Microcirculation (New York, N.Y. : 1994) · 2011Article
- Intravital microscopy: new insights into metastasis of tumors.Journal of cell science · 2011Review
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Authors and funding
5 authors at 1 institution in 1 country.
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Abstract
The cellular mechanisms that control arterial diameter in vivo, particularly in hypertension, are uncertain. Here, we report a method that permits arterial intracellular Ca(2+) concentration ([Ca(2+)](i)), myosin light-chain kinase (MLCK) activation, and artery external diameter to be recorded simultaneously with arterial blood pressure (BP) in living mice under 1.5% isofluorane anesthesia. The method also enables an assessment of local receptor activity on [Ca(2+)](i), MLCK activity, and diameter in arteries, uncomplicated by systemic effects. Transgenic mice that express, in smooth muscle, a Ca(2+)/calmodulin-activated, Förster resonance energy transfer (FRET)-based "ratiometric", exogenous MLCK biosensor were used. Vasoactive substances were administered either intravenously or locally to segments of exposed femoral or cremaster arteries. In the basal state, mean BP was approximately 90 mmHg, femoral arteries were constricted to 65% of their passive diameter, MLCK fractional activation was 0.14, and [Ca(2+)](i) was 131 nM. Phenylephrine (300 ng/g wt iv) elevated mean BP transiently to approximately 110 mmHg, decreased heart rate, increased femoral artery [Ca(2+)](i) to 244 nM and fractional MLCK activation to 0.24, and decreased artery diameter by 23%. In comparison, local application of 1.0 muM phenylephrine raised [Ca(2+)](i) to 279 nM and fractional MLCK activation to 0.26, and reduced diameter by 25%, but did not affect BP or heart rate. Intravital FRET imaging of exogenous MLCK biosensor mice permits quantification of changes in [Ca(2+)](i) and MLCK activation that accompany small changes in BP. Based on the observed variance of the FRET data, this method should enable the detection of a difference in basal [Ca(2+)](i) of 29 nM between two groups of 12 mice with a significance of P < 0.05.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.