Evidence map›Paper›PMID 20622068›Full record

ArticleJournal of bacteriology2010

Functional identification of the Proteus mirabilis core lipopolysaccharide biosynthesis genes.

Eleonora Aquilini, Joana Azevedo, Natalia Jimenez, Lamiaa Bouamama, Juan M Tomás, Miguel Regué

Open access · greenAbstract read
In one paragraph

Article in Journal of bacteriology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
1.2field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 20 citations in OpenAlex.

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    Article
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  11. Article
  12. Article
  13. Article
  14. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Eleonora AquiliniDepartamento Microbiología y Parasitología Sanitarias, Facultad de Farmacia, Universidad Barcelona, Av. Joan XXIII s/n, Barcelona, Spain.
Joana Azevedo
Natalia Jimenez
Lamiaa Bouamama
Juan M Tomás
Miguel Regué
Universitat de Barcelona · ES

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In this study, we report the identification of genes required for the biosynthesis of the core lipopolysaccharides (LPSs) of two strains of Proteus mirabilis. Since P. mirabilis and Klebsiella pneumoniae share a core LPS carbohydrate backbone extending up to the second outer-core residue, the functions of the common P. mirabilis genes was elucidated by genetic complementation studies using well-defined mutants of K. pneumoniae. The functions of strain-specific outer-core genes were identified by using as surrogate acceptors LPSs from two well-defined K. pneumoniae core LPS mutants. This approach allowed the identification of two new heptosyltransferases (WamA and WamC), a galactosyltransferase (WamB), and an N-acetylglucosaminyltransferase (WamD). In both strains, most of these genes were found in the so-called waa gene cluster, although one common core biosynthetic gene (wabO) was found outside this cluster.

Indexed as

Multigene FamilyBacterial ProteinsCarbohydrate SequenceGalactosyltransferasesGenetic Complementation TestGlucosyltransferasesGlycosyltransferasesKlebsiella pneumoniaeLipopolysaccharidesMolecular Sequence DataMutationN-AcetylglucosaminyltransferasesProteus mirabilisSequence Analysis, DNABacterial ProteinsGalactosyltransferasesGlucosyltransferasesGlycosyltransferasesheptosyltransferaselipopolysaccharide, ProteusLipopolysaccharidesN-AcetylglucosaminyltransferasesN-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase

Identifiers

PMID20622068
PMCPMC2937370
OpenAlexW2107014578

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.