ArticleJournal of bacteriology2010
Systems-level metabolic flux profiling elucidates a complete, bifurcated tricarboxylic acid cycle in Clostridium acetobutylicum.
Article in Journal of bacteriology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed.
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- Comparative Metabolomics ofJournal of microbiology and biotechnology · 2025Article
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- The gut microbiome of farmed Arctic char (FEMS microbes · 2024Article
- Systems biology elucidates the distinctive metabolic niche filled by the human gut microbe Eggerthella lenta.PLoS biology · 2023Article
- Co‑cultivation of anaerobic fungi with Clostridium acetobutylicum bolsters butyrate and butanol production from cellulose and lignocellulose.Journal of industrial microbiology & biotechnology · 2023Article
- Soil Metabolomics Predict Microbial Taxa as Biomarkers of Moisture Status in Soils from a Tidal Wetland.Microorganisms · 2022Article
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- A thermodynamic atlas of carbon redox chemical space.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Glucose transporter 1 is important for the glycolytic metabolism of human endometrial stromal cells in hypoxic environment.Heliyon · 2020Article
- Genome-Scale Metabolic Network Reconstruction and In Silico Analysis of Hexanoic acid ProducingMicroorganisms · 2020Article
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- Metabolomics Exploration of Pseudorabies Virus Reprogramming Metabolic Profiles of PK-15 Cells to Enhance Viral Replication.Frontiers in cellular and infection microbiology · 2020Article
- The Stringent Response Determines the Ability of a Commensal Bacterium to Survive Starvation and to Persist in the Gut.Cell host & microbe · 2018Article
- Enhanced isopropanol-butanol-ethanol mixture production through manipulation of intracellular NAD(P)H level in the recombinantBiotechnology for biofuels · 2018Article
- Selection of fast and slow growing bacteria from fecal microbiota using continuous culture with changing dilution rate.Microbial ecology in health and disease · 2018Article
- Isotope-Assisted Metabolite Analysis Sheds Light on Central Carbon Metabolism of a Model Cellulolytic BacteriumFrontiers in microbiology · 2018Article
- Amino acid catabolism-directed biofuel production inBiotechnology reports (Amsterdam, Netherlands) · 2017Review
- Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Obligatory anaerobic bacteria are major contributors to the overall metabolism of soil and the human gut. The metabolic pathways of these bacteria remain, however, poorly understood. Using isotope tracers, mass spectrometry, and quantitative flux modeling, here we directly map the metabolic pathways of Clostridium acetobutylicum, a soil bacterium whose major fermentation products include the biofuels butanol and hydrogen. While genome annotation suggests the absence of most tricarboxylic acid (TCA) cycle enzymes, our results demonstrate that this bacterium has a complete, albeit bifurcated, TCA cycle; oxaloacetate flows to succinate both through citrate/alpha-ketoglutarate and via malate/fumarate. Our investigations also yielded insights into the pathways utilized for glucose catabolism and amino acid biosynthesis and revealed that the organism's one-carbon metabolism is distinct from that of model microbes, involving reversible pyruvate decarboxylation and the use of pyruvate as the one-carbon donor for biosynthetic reactions. This study represents the first in vivo characterization of the TCA cycle and central metabolism of C. acetobutylicum. Our results establish a role for the full TCA cycle in an obligatory anaerobic organism and demonstrate the importance of complementing genome annotation with isotope tracer studies for determining the metabolic pathways of diverse microbes.
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