ArticleThe Journal of biological chemistry2010
Transcriptomic analyses of xylan degradation by Prevotella bryantii and insights into energy acquisition by xylanolytic bacteroidetes.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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Who cites it
65 citing papers in PubMed, 143 citations in OpenAlex.
- Attapulgite-enhanced probiotic delivery reprograms gut microbiota and metabolism to improve growth performance and immune function in broilers.Poultry science · 2026Article
- Rumen-derived Prevotella and Megasphaera elsdenii mitigate methane production through functional modulation of rumen microbial metabolism.Journal of animal science and biotechnology · 2026Article
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- Effects ofAnimals : an open access journal from MDPI · 2026Article
- Improving beef quality through rumen microbial modulation by varying alfalfa hay proportions in the diet.Frontiers in microbiology · 2026Article
- Complete genome sequence ofMicrobiology resource announcements · 2025Article
- ez-CAZy a reference annotation database for linking glycoside hydrolase sequence to enzymatic activity.Scientific reports · 2025Article
- Transcriptional delineation of polysaccharide utilization loci in the human gut commensalApplied and environmental microbiology · 2025Article
- Complete xylan utilization pathway and regulation mechanisms involved in marine algae degradation by cosmopolitan marine and human gut microbiota.The ISME journal · 2025Article
- Transcriptomic analysis of polysaccharide utilization loci reveals substrate preferences in ruminal generalists Segatella bryantii TF1-3 and Xylanibacter ruminicola KHP1.BMC genomics · 2024Article
- Effects of Isochlorogenic Acid on Ewes Rumen Fermentation, Microbial Diversity and Ewes Immunity of Different Physiological Stages.Animals : an open access journal from MDPI · 2024Article
- The catabolic specialization of the marine bacteriumApplied and environmental microbiology · 2024Article
- Altered rumen microbiome and correlations of the metabolome in heat-stressed dairy cows at different growth stages.Microbiology spectrum · 2023Article
- HadzaCell reports · 2023Article
- HadzabioRxiv : the preprint server for biology · 2023Article
- Prevotella: A Key Player in Ruminal Metabolism.Microorganisms · 2022Review
- Increasing buffering capacity enhances rumen fermentation characteristics and alters rumen microbiota composition of high-concentrate fed Hanwoo steers.Scientific reports · 2022Article
- Role of Bifidobacterium pseudocatenulatum in Degradation and Consumption of Xylan-Derived Carbohydrates.Applied and environmental microbiology · 2022Article
- Article
- A Combination of Novel Microecological Agents and Molasses Role in Digestibility and Fermentation of Rice Straw by Facilitating the Ruminal Microbial Colonization.Frontiers in microbiology · 2022Article
5 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Enzymatic depolymerization of lignocellulose by microbes in the bovine rumen and the human colon is critical to gut health and function within the host. Prevotella bryantii B(1)4 is a rumen bacterium that efficiently degrades soluble xylan. To identify the genes harnessed by this bacterium to degrade xylan, the transcriptomes of P. bryantii cultured on either wheat arabinoxylan or a mixture of its monosaccharide components were compared by DNA microarray and RNA sequencing approaches. The most highly induced genes formed a cluster that contained putative outer membrane proteins analogous to the starch utilization system identified in the prominent human gut symbiont Bacteroides thetaiotaomicron. The arrangement of genes in the cluster was highly conserved in other xylanolytic Bacteroidetes, suggesting that the mechanism employed by xylan utilizers in this phylum is conserved. A number of genes encoding proteins with unassigned function were also induced on wheat arabinoxylan. Among these proteins, a hypothetical protein with low similarity to glycoside hydrolases was shown to possess endoxylanase activity and subsequently assigned to glycoside hydrolase family 5. The enzyme was designated PbXyn5A. Two of the most similar proteins to PbXyn5A were hypothetical proteins from human colonic Bacteroides spp., and when expressed each protein exhibited endoxylanase activity. By using site-directed mutagenesis, we identified two amino acid residues that likely serve as the catalytic acid/base and nucleophile as in other GH5 proteins. This study therefore provides insights into capture of energy by xylanolytic Bacteroidetes and the application of their enzymes as a resource in the biofuel industry.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.