ArticleJournal of the American Society for Mass Spectrometry2010
The study of protein conformation in solution via direct sampling by desorption electrospray ionization mass spectrometry.
Article in Journal of the American Society for Mass Spectrometry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 51 citations in OpenAlex.
- Time-Resolved Native Mass Spectrometry for Direct Measurement of Biomolecular Kinetics.Journal of the American Chemical Society · 2026Article
- Simple Protein Analysis by Droplet Paper Spray Ionization Mass Spectrometry with Polyolefin Silica-Based Paper.Molecules (Basel, Switzerland) · 2023Article
- Direct Evidence for the Origin of Bis-Gold Intermediates: Probing Gold Catalysis with Mass Spectrometry.Chemistry (Weinheim an der Bergstrasse, Germany) · 2018Article
- Online Monitoring of Methanol Electro-Oxidation Reactions by Ambient Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2017Article
- Enhancing Performance of Liquid Sample Desorption Electrospray Ionization Mass Spectrometry Using Trap and Capillary Columns.International journal of mass spectrometry · 2015Article
- Paper-Based Electrochemical Cell Coupled to Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2015Article
- Online Investigation of Aqueous-Phase Electrochemical Reactions by Desorption Electrospray Ionization Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2015Article
- Integration of electrochemistry with ultra-performance liquid chromatography/mass spectrometry.European journal of mass spectrometry (Chichester, England) · 2015Article
- Quantifying protein-carbohydrate interactions using liquid sample desorption electrospray ionization mass spectrometry.Journal of the American Society for Mass Spectrometry · 2015Article
- A new splitting method for both analytical and preparative LC/MS.Journal of the American Society for Mass Spectrometry · 2014Article
- Super-Atmospheric Pressure Ion Sources: Application and Coupling to API Mass Spectrometer.Mass spectrometry (Tokyo, Japan) · 2014Article
- Measuring protein-ligand interactions using liquid sample desorption electrospray ionization mass spectrometry.Analytical chemistry · 2013Article
- Integration of online digestion and electrolytic reduction with mass spectrometry for rapid disulfide-containing protein structural analysis.International journal of mass spectrometry · 2013Article
- Protein structure evolution in liquid DESI as revealed by selective noncovalent adduct protein probing.International journal of mass spectrometry · 2012Article
- Investigation of some biologically relevant redox reactions using electrochemical mass spectrometry interfaced by desorption electrospray ionization.Analytical and bioanalytical chemistry · 2012Article
- Fast and selective modification of thiol proteins/peptides by N-(phenylseleno)phthalimide.Journal of the American Society for Mass Spectrometry · 2012Article
- Transient Ru-methyl formate intermediates generated with bifunctional transfer hydrogenation catalysts.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Reflections on charge state distributions, protein structure, and the mystical mechanism of electrospray ionization.Journal of the American Society for Mass Spectrometry · 2012Article
- Direct ionization of large proteins and protein complexes by desorption electrospray ionization-mass spectrometry.Analytical chemistry · 2011Article
- Direct detection of pharmaceuticals and personal care products from aqueous samples with thermally-assisted desorption electrospray ionization mass spectrometry.Journal of the American Society for Mass Spectrometry · 2011Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The direct sampling feature of liquid sample desorption electrospray ionization (DESI) allows the ionization of liquid samples without adding acids/organic solvents (i.e., without sample pretreatment). As a result, it provides a new approach for probing protein conformation in solution. In this study, it has been observed that native protein ions are generated from proteins in water by DESI. Interestingly, the intensities of the resulting protein ions appear to be higher than those generated by ESI of the proteins in water or in ammonium acetate. For protein solutions that already contain acids/organic solvents, DESI can be used to investigate the influences of these denaturants on protein conformations and the obtained results are in good agreement with spectroscopic data. In addition, online monitoring of protein conformational changes by DESI is feasible; for instance, heat-induced unfolding of ubiquitin can be traced with DESI in water without influences of organic solvents/acids. This DESI method provides a new alternative tool for the study of protein conformation in solution.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.