ArticleFungal genetics and biology : FG & B2011
Characterization of the GPI-anchored endo β-1,3-glucanase Eng2 of Aspergillus fumigatus.
Article in Fungal genetics and biology : FG & B, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
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- Novel, cold-adapted D-laminaribiose- and D-glucose-releasing GH16 endo-β-1,3-glucanase fromFrontiers in microbiology · 2024Article
- Novel Insights into the Mechanism Underlying High Polysaccharide Yield in Submerged Culture ofMicroorganisms · 2023Article
- Characterization ofCell surface (Amsterdam, Netherlands) · 2022Article
- From Cancer Therapy to Winemaking: The Molecular Structure and Applications of β-Glucans and β-1, 3-Glucanases.International journal of molecular sciences · 2022Review
- Calcineurin Regulates Conidiation, Chlamydospore Formation and Virulence inFrontiers in microbiology · 2020Article
- Glucanase-Induced Stipe Wall Extension Shows Distinct Differences from Chitinase-Induced Stipe Wall Extension of Coprinopsis cinerea.Applied and environmental microbiology · 2019Article
- The Genetics and Biochemistry of Cell Wall Structure and Synthesis inFrontiers in microbiology · 2019Review
- Ceratocystis cacaofunesta genome analysis reveals a large expansion of extracellular phosphatidylinositol-specific phospholipase-C genes (PI-PLC).BMC genomics · 2018Article
- Ligation of Dectin-2 with a novel microbial ligand promotes adjuvant activity for vaccination.PLoS pathogens · 2017Article
- Characterization of the Sclerotinia sclerotiorum cell wall proteome.Molecular plant pathology · 2016Article
- Recent advances in the understanding of the Aspergillus fumigatus cell wall.Journal of microbiology (Seoul, Korea) · 2016Review
- β-1,3-glucan modifying enzymes in Aspergillus fumigatus.Frontiers in microbiology · 2013Article
- Article
- A novel glycosylphosphatidylinositol-anchored glycoside hydrolase from Ustilago esculenta functions in β-1,3-glucan degradation.Applied and environmental microbiology · 2012Article
- Characterization of PbPga1, an antigenic GPI-protein in the pathogenic fungus Paracoccidioides brasiliensis.PloS one · 2012Article
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4 authors.
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Abstract
A GPI-anchored endo β-1,3-glucanase of Aspergillus fumigatus was characterized. The enzyme encoded by ENG2 (AFUA_2g14360) belongs to the glycoside hydrolase family 16 (GH16). The activity was characterized using a recombinant protein produced by Pichiapastoris. The recombinant enzyme preferentially acts on soluble β-1,3-glucans. Enzymatic analysis of the endoglucanase activity using Carboxymethyl-Curdlan-Remazol Brilliant Blue (CM-Curdlan-RBB) as a substrate revealed a wide temperature optimum of 24-40°C, a pH optimum of 5.0-6.5 and a K(m) of 0.8 mg ml(-1). HPAEC analysis of the products formed by Eng2 when acting on different oligo-β-1,3-glucans confirmed the predicted endoglucanase activity and also revealed a transferase activity for oligosaccharides of a low degree of polymerization. The growth phenotype of the Afeng2 mutant was identical to that of the wt strain.
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