ArticleMolecular plant pathology2010
Molecular characterization of a serine protease Pro1 from Plasmodiophora brassicae that stimulates resting spore germination.
Article in Molecular plant pathology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 79 citations in OpenAlex.
- The clubroot pathogen Plasmodiophora brassicae: A profile update.Molecular plant pathology · 2023Review
- Genome-Wide Identification and Analysis of the Maize Serine Peptidase S8 Family Genes in Response to Drought at Seedling Stage.Plants (Basel, Switzerland) · 2023Article
- Advances in Biological Control and Resistance Genes of Brassicaceae Clubroot Disease-The Study Case of China.International journal of molecular sciences · 2023Review
- The current status, challenges, and future perspectives for managing diseases of brassicas.Frontiers in microbiology · 2023Review
- Identification of Pathogenicity-Related Effector Proteins and the Role of Piwsc1 in the Virulence ofJournal of fungi (Basel, Switzerland) · 2022Article
- What Can We Learn from -Omics Approaches to Understand Clubroot Disease?International journal of molecular sciences · 2022Review
- Looking for a Cultured Surrogate for Effectome Studies of the Clubroot Pathogen.Frontiers in microbiology · 2021Article
- Candidate Effectors ofFrontiers in microbiology · 2021Article
- Methods for Assessment of Viability and Germination ofFrontiers in microbiology · 2021Article
- Transcriptome Analysis Identifies Plasmodiophora brassicae Secondary Infection Effector Candidates.The Journal of eukaryotic microbiology · 2020Article
- Genome-wide identification of genes encoding putative secreted E3 ubiquitin ligases and functional characterization of PbRING1 in the biotrophic protist Plasmodiophora brassicae.Current genetics · 2019Article
- Transcriptomic response in symptomless roots of clubroot infected kohlrabi (Brassica oleracea var. gongylodes) mirrors resistant plants.BMC plant biology · 2019Article
- Comparative Transcriptome Analysis Reveals Key Pathways and Hub Genes in Rapeseed During the Early Stage ofFrontiers in genetics · 2019Article
- Proto-oncogenes in a eukaryotic unicellular organism play essential roles in plasmodial growth in host cells.BMC genomics · 2018Article
- Review
- A novel methyltransferase from the intracellular pathogen Plasmodiophora brassicae methylates salicylic acid.Molecular plant pathology · 2015Article
- A putative serine protease, SpSsp1, from Saprolegnia parasitica is recognised by sera of rainbow trout, Oncorhynchus mykiss.Fungal biology · 2014Article
- Plasmodiophora brassicae: a review of an emerging pathogen of the Canadian canola (Brassica napus) crop.Molecular plant pathology · 2012Review
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Clubroot, caused by Plasmodiophora brassicae, is one of the most serious diseases of cultivated cruciferous crops in the world. However, the basis for pathogenicity in P. brassicae is not well understood. In this study, a serine protease gene (PRO1) was cloned from P. brassicae and its molecular characteristics were investigated. Southern analysis and specific polymerase chain reaction (PCR) amplification indicated that PRO1 is a single-copy gene present in a broad range of P. brassicae pathotypes. Northern analysis revealed that the expression of PRO1 was induced during plant infection, and that the quantity of transcript fluctuated according to the stage of pathogenesis. Amino acid sequence analysis suggested that the encoded protein (Pro1) belongs to the S28 family of proteases, with a predicted signal peptide and a theoretical molecular mass of 49.4 kDa. The open reading frame (ORF) of PRO1 was transferred into Pichia pastoris and Pro1 was heterologously produced. Pro1 showed proteolytic activity on skimmed milk and N-succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin, and the activity could be inhibited by serine protease inhibitors and the chelating agent ethylenediaminetetraacetic acid. The optimal temperature of Pro1 was 25 degrees C, and it exhibited high activity at pH 6.0-6.4. These values coincide with the temperature and pH conditions favourable for P. brassicae resting spore germination in the field. When Pro1 was used to treat canola root exudates, it enhanced the stimulating effect of the root exudates on P. brassicae resting spore germination, indicating that Pro1 may play a role during clubroot pathogenesis by stimulating resting spore germination through its proteolytic activity.
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