ArticleMolecular plant pathology2010
The autophagy genes ATG8 and ATG1 affect morphogenesis and pathogenicity in Ustilago maydis.
Article in Molecular plant pathology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers, 3 of them syntheses that pooled it.
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37 citing papers in PubMed, 3 syntheses or guidelines pooled it, 76 citations in OpenAlex.
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- Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition).Autophagy · 2016Guideline
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- Optimization and scale up of L-malic acid production from methanol by the methylotrophic yeast Ogataea polymorpha.FEMS yeast research · 2026Article
- Transcriptomic and metabolomic analyses unravel the different pathogenic mechanisms ofFrontiers in microbiology · 2025Article
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- Autophagy Is Required to Sustain Increased Intestinal Cell Proliferation during Phenotypic Plasticity Changes in Honey Bee (International journal of molecular sciences · 2023Article
- Pexophagy is critical for fungal development, stress response, and virulence in Alternaria alternata.Molecular plant pathology · 2022Article
- Autophagy-Related GeneInternational journal of molecular sciences · 2022Article
- Vacuolar proteases and autophagy in phytopathogenic fungi: A review.Frontiers in fungal biology · 2022Review
- The crucial role of the regulatory mechanism of the Atg1/ULK1 complex in fungi.Frontiers in microbiology · 2022Review
- Vacuolar Protein-Sorting Receptor MoVps13 Regulates Conidiation and Pathogenicity in Rice Blast FungusJournal of fungi (Basel, Switzerland) · 2021Article
- A VASt-domain protein regulates autophagy, membrane tension, and sterol homeostasis in rice blast fungus.Autophagy · 2021Article
- Functional Analysis of Autophagy-Related GeneInternational journal of molecular sciences · 2021Article
- A fungal member of theeLife · 2021Article
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- Identifying Genes Devoted to the Cell Death Process in the Gene Regulatory Network ofFrontiers in microbiology · 2021Article
- UvAtg8-Mediated Autophagy Regulates Fungal Growth, Stress Responses, Conidiation, and Pathogenesis in Ustilaginoidea virens.Rice (New York, N.Y.) · 2020Article
- Biological functions of the autophagy-related proteins Atg4 and Atg8 in Cryptococcus neoformans.PloS one · 2020Article
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Authors and funding
2 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Autophagy is a complex degradative process in which cytosolic material, including organelles, is randomly sequestered within double-membrane vesicles termed autophagosomes. In Saccharomyces cerevisiae, the autophagy genes ATG1 and ATG8 are crucial for autophagy induction and autophagosome assembly, respectively, and their deletion has an impact on the autophagic potential of the corresponding mutant strains. We were interested in the role of autophagy in the development and virulence of U. maydis. Using a reverse genetic approach, we showed that the U. maydis ATG8 orthologue, atg8, is associated with autophagy-dependent processes. Deletion of atg8 abolished autophagosome accumulation in the vacuoles of carbon-starved cells and drastically reduced the survival of U. maydisDeltaatg8 mutant strains during these conditions. In addition, atg8 deletion had an impact on the budding process during saprobic haploid growth. The infection of maize with compatible Deltaatg8 strains resulted in fewer galled plants, and fungal gall colonization was strongly reduced, as reflected by the very low hyphal density in these tissues. Deltaatg8 infections resulted in the formation of very few teliospores. To corroborate the role of autophagy in U. maydis development, we also deleted the ATG1 orthologue, atg1. Deletion of atg1 yielded phenotypes similar to the Deltaatg8 strains during saprobic growth, but of lower magnitude. The Deltaatg1 strains were only slightly less pathogenic than the wild-type and teliospore production was not affected. Surprisingly, atg1 deletion in the Deltaatg8 background exacerbated those phenotypes already observed in the Deltaatg8 and Deltaatg1 single-mutant strains, strongly suggesting an additive phenotype. In particular, the double mutant was completely suppressed for plant gall induction.
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