ArticleTissue engineering. Part A2010
Effect of phosphatidyl inositol 3-kinase, extracellular signal-regulated kinases 1/2, and p38 mitogen-activated protein kinase inhibition on osteogenic differentiation of muscle-derived stem cells.
Article in Tissue engineering. Part A, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 38 citations in OpenAlex.
- KLF9 regulates osteogenic differentiation of mesenchymal stem cells.Journal of molecular histology · 2024Article
- Dentin-Derived Inorganic Minerals Promote the Osteogenesis of Bone Marrow-Derived Mesenchymal Stem Cells: Potential Applications for Bone Regeneration.Stem cells international · 2020Article
- Muscle-bone crosstalk and potential therapies for sarco-osteoporosis.Journal of cellular biochemistry · 2019Review
- Negative pressure wound therapy promotes muscle-derived stem cell osteogenic differentiation through MAPK pathway.Journal of cellular and molecular medicine · 2018Article
- An investigation of BMP-7 mediated alterations to BMP signalling components in human tenocyte-like cells.Scientific reports · 2016Article
- Stem cell death and survival in heart regeneration and repair.Apoptosis : an international journal on programmed cell death · 2016Review
- Impact of the source and serial passaging of goat mesenchymal stem cells on osteogenic differentiation potential: implications for bone tissue engineering.Journal of animal science and biotechnology · 2016Article
- Activation of the Extracellular Signal-Regulated Kinase Signaling Is Critical for Human Umbilical Cord Mesenchymal Stem Cell Osteogenic Differentiation.BioMed research international · 2016Article
- Extracellular regulated protein kinases 1/2 phosphorylation is required for hepatic differentiation of human umbilical cord-derived mesenchymal stem cells.Experimental biology and medicine (Maywood, N.J.) · 2015Article
- Silica nanoparticles increase human adipose tissue-derived stem cell proliferation through ERK1/2 activation.International journal of nanomedicine · 2015Article
- The p38α MAPK function in osteoprecursors is required for bone formation and bone homeostasis in adult mice.PloS one · 2014Article
- Scaffold-free microtissues: differences from monolayer cultures and their potential in bone tissue engineering.Clinical oral investigations · 2013Review
- BMP9 signaling in stem cell differentiation and osteogenesis.American journal of stem cells · 2013Article
- Matrix metalloproteinase inhibition negatively affects muscle stem cell behavior.International journal of clinical and experimental pathology · 2013Article
- P38 and ERK1/2 MAPKs act in opposition to regulate BMP9-induced osteogenic differentiation of mesenchymal progenitor cells.PloS one · 2012Article
- p85alpha regulates osteoblast differentiation by cross-talking with the MAPK pathway.The Journal of biological chemistry · 2011Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Skeletal muscle-derived stem cells (MDSCs) can undergo osteogenesis when treated with bone morphogenetic proteins (BMPs), making them a potential cell source for bone tissue engineering. The signaling pathways that regulate BMP4-induced osteogenesis in MDSCs are not well understood, although they may provide a means to better regulate differentiation during bone regeneration. The objective of this study was to characterize the signaling pathways involved in the BMP4-induced osteogenesis of MDSCs. Cells were treated with BMP4 and specific inhibitors to the extracellular signal-regulated kinases 1/2 (ERK1/2), p38 mitogen-activated protein kinase (MAPK), and phosphatidyl inositol 3-kinase (PI3K) pathways (PD98059, SB203580, and Ly294002, respectively). Cellular proliferation, expression of osteoblast-related genes, alkaline phosphatase (ALP) activity, and tissue mineralization were measured to determine the role of each pathway in the osteogenic differentiation of MDSCs. Inhibition of the ERK1/2 pathway increased ALP activity and mineralization, whereas inhibition of the p38 MAPK pathway decreased osteogenesis, suggesting opposing roles of these pathways in the BMP4-induced osteogenesis of MDSCs. Inhibition of the PI3K pathway significantly increased mineralization by MDSCs. These findings highlight the involvement of the ERK1/2, p38 MAPK, and PI3K pathways in opposing capacities in MDSC differentiation and warrant further investigation, as it may identify novel therapeutic targets for the development of stem cell-based therapies for bone tissue engineering.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.