ArticleAnalytical chemistry2010
Microfluidic system for generation of sinusoidal glucose waveforms for entrainment of islets of Langerhans.
Article in Analytical chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 52 citations in OpenAlex.
- Automated and online LC-MS/MS for multiplexed monitoring of hormone secretion from murine islets.Analytical and bioanalytical chemistry · 2026Article
- Glucose-driven intra- and inter-islet beta cell synchronization in pancreatic tissue slices.Scientific reports · 2026Article
- Droplet-based μChopper device with a 3D-printed pneumatic valving layer and a simple photometer for absorbance based fructosamine quantification in human serum.The Analyst · 2023Article
- Visualizing hypoxic modulation of beta cell secretions via a sensor augmented oxygen gradient.Microsystems & nanoengineering · 2023Article
- An automated do-it-yourself system for dynamic stem cell and organoid culture in standard multi-well plates.Cell reports methods · 2022Article
- Pancreatic islet organoids-on-a-chip: how far have we gone?Journal of nanobiotechnology · 2022Review
- Islet-on-a-chip for the study of pancreatic β-cell function.In vitro models · 2022Review
- Building Biomimetic Potency Tests for Islet Transplantation.Diabetes · 2021Review
- Synchronized stimulation and continuous insulin sensing in a microfluidic human Islet on a Chip designed for scalable manufacturing.Lab on a chip · 2019Article
- Microfluidic device for real-time formulation of reagents and their subsequent encapsulation into double emulsions.Scientific reports · 2018Article
- A microfluidic in-line ELISA for measuring secreted protein under perfusion.Biomedical microdevices · 2017Article
- Online fluorescence anisotropy immunoassay for monitoring insulin secretion from islets of Langerhans.Analytical methods : advancing methods and applications · 2017Article
- A Y-Shaped Microfluidic Device to Study the Combined Effect of Wall Shear Stress and ATP Signals on Intracellular Calcium Dynamics in Vascular Endothelial Cells.Micromachines · 2016Article
- Dual Detection System for Simultaneous Measurement of Intracellular Fluorescent Markers and Cellular Secretion.Analytical chemistry · 2016Article
- Tracking matrix effects in the analysis of DNA adducts of polycyclic aromatic hydrocarbons.Journal of chromatography. A · 2016Article
- Measurement of the entrainment window of islets of Langerhans by microfluidic delivery of a chirped glucose waveform.Integrative biology : quantitative biosciences from nano to macro · 2015Article
- Microfluidic platform for assessing pancreatic islet functionality through dielectric spectroscopy.Biomicrofluidics · 2015Article
- An automated programmable platform enabling multiplex dynamic stimuli delivery and cellular response monitoring for high-throughput suspension single-cell signaling studies.Lab on a chip · 2015Article
- Integrated perfusion and separation systems for entrainment of insulin secretion from islets of Langerhans.Lab on a chip · 2015Article
- Negative feedback synchronizes islets of Langerhans.Biophysical journal · 2014Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
A microfluidic system was developed to produce sinusoidal waveforms of glucose to entrain oscillations of intracellular [Ca(2+)] in islets of Langerhans. The work described is an improvement to a previously reported device where two pneumatic pumps delivered pulses of glucose and buffer to a mixing channel. The mixing channel acted as a low pass filter to attenuate these pulses to produce the desired final concentration. Improvements to the current device included increasing the average pumping frequency from 0.83 to 3.33 Hz by modifying the on-chip valves to minimize adhesion between the PDMS and glass within the valve. The cutoff frequency of the device was increased from 0.026 to 0.061 Hz for sinusoidal fluorescein waves by shortening the length of the mixing channel to 3.3 cm. The value of the cutoff frequency was chosen between the average pumping frequency and the low frequency (approximately 0.0056 Hz) glucose waves that were needed to entrain the islets of Langerhans. In this way, the pulses from the pumps were attenuated greatly but the low-frequency glucose waves were not. With the use of this microfluidic system, a total flow rate of 1.5 +/- 0.1 microL min(-1) was generated and used to deliver sinusoidal waves of glucose concentration with a median value of 11 mM and amplitude of 1 mM to a chamber that contained an islet of Langerhans loaded with the Ca(2+)-sensitive fluorophore, indo-1. Entrainment of the islets was demonstrated by pacing the rhythm of intracellular [Ca(2+)] oscillations to oscillatory glucose levels produced by the device. The system should be applicable to a wide range of cell types to aid understanding cellular responses to dynamically changing stimuli.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.