ArticleThe Analyst2010
A PCR reactor with an integrated alumina membrane for nucleic acid isolation.
Article in The Analyst, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed.
- Two-step method for rapid isolation of genomic DNA and validation of R81T insecticide resistance mutation inSaudi journal of biological sciences · 2023Article
- Article
- Membrane Technology for Rapid Point-of-Care Diagnostics for Parasitic Neglected Tropical Diseases.Clinical microbiology reviews · 2021Article
- Sample Preparation and Diagnostic Methods for a Variety of Settings: A Comprehensive Review.Molecules (Basel, Switzerland) · 2021Review
- An Overview on Microfluidic Systems for Nucleic Acids Extraction from Human Raw Samples.Sensors (Basel, Switzerland) · 2021Review
- Advances in point-of-care nucleic acid extraction technologies for rapid diagnosis of human and plant diseases.Biosensors & bioelectronics · 2020Review
- Rapid (30-second), equipment-free purification of nucleic acids using easy-to-make dipsticks.Nature protocols · 2020Article
- Review
- Fast DNA Extraction with Polyacrylamide Microspheres for Polymerase Chain Reaction Detection.ACS omega · 2020Article
- A Novel Microfluidic Device Integrated with Chitosan-Modified Capillaries for Rapid ZIKV Detection.Micromachines · 2020Article
- Swift Large-scale Examination of Directed Genome Editing.PloS one · 2019Article
- Article
- Miniaturized devices for point of care molecular detection of HIV.Lab on a chip · 2017Review
- Combining Electro-Osmotic Flow and FTAMicromachines · 2016Article
- Microfluidic devices for nucleic acid (NA) isolation, isothermal NA amplification, and real-time detection.Methods in molecular biology (Clifton, N.J.) · 2015Article
- A microfluidic chip integrating DNA extraction and real-time PCR for the detection of bacteria in saliva.Lab on a chip · 2013Article
- Towards a "Sample-In, Answer-Out" Point-of-Care Platform for Nucleic Acid Extraction and Amplification: Using an HPV E6/E7 mRNA Model System.Journal of oncology · 2012Article
- Point-of-care oral-based diagnostics.Oral diseases · 2011Review
- An isothermal amplification reactor with an integrated isolation membrane for point-of-care detection of infectious diseases.The Analyst · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
Recently, there has been a growing interest in point-of-care devices capable of detecting nucleic acids (NA) in clinical and environmental samples. Nucleic acid detection requires, however, various sample preparation steps that complicate device operation. An attractive remedy is to integrate many, if not all, sample preparation operations and nucleic acid amplification into a single reaction chamber. A microfluidic chip that integrates, in a single chamber, polymerase chain reaction (PCR) amplification with solid-phase extraction of nucleic acids using a nanoporous, aluminium oxide membrane (AOM) is described. Samples suspected of containing target bacteria and/or viruses are mixed with lysis agents and a chaotropic salt and loaded into a plastic chip housing a nanoporous, aluminium oxide membrane. The nucleic acids in the lysate bind to the membrane. The membrane is then washed, the chamber is filled with the PCR reaction reagents, and the chamber's temperature is cycled to amplify the captured nucleic acids and produce detectable products. Both DNA and RNA (with reverse-transcription) isolation and amplification are demonstrated. Due to the dry membrane's high resistance to liquid flow, a specialized flow control system was devised to facilitate sample introduction and membrane washing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.