ArticleJournal of lipid research2010
Lipid mapping of colonic mucosa by cluster TOF-SIMS imaging and multivariate analysis in cftr knockout mice.
Article in Journal of lipid research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 62 citations in OpenAlex.
- High-Resolution Microscope-Mode Secondary Ion Mass Spectrometry Imaging.Analytical chemistry · 2026Article
- The Application of a Random Forest Classifier to ToF-SIMS Imaging Data.Journal of the American Society for Mass Spectrometry · 2024Article
- Review
- SEAM is a spatial single nuclear metabolomics method for dissecting tissue microenvironment.Nature methods · 2021Article
- Cryogenic OrbiSIMS Localizes Semi-Volatile Molecules in Biological Tissues.Angewandte Chemie (International ed. in English) · 2020Article
- ToF-SIMS and Machine Learning for Single-Pixel Molecular Discrimination of an Acrylate Polymer Microarray.Analytical chemistry · 2020Article
- Unsupervised machine learning for exploratory data analysis in imaging mass spectrometry.Mass spectrometry reviews · 2020Review
- Correlative mass spectrometry imaging, applying time-of-flight secondary ion mass spectrometry and atmospheric pressure matrix-assisted laser desorption/ionization to a single tissue section.Rapid communications in mass spectrometry : RCM · 2018Article
- Reduce the matrix effect in biological tissue imaging using dynamic reactive ionization and gas cluster ion beams.Biointerphases · 2016Article
- Chemometric analysis of MALDI mass spectrometric images of three-dimensional cell culture systems.Analytical methods : advancing methods and applications · 2015Article
- Mass spectrometry coupled to imaging techniques: the better the view the greater the challenge.Frontiers in physiology · 2015Article
- Image and Spectral Processing for ToF-SIMS Analysis of Biological Materials.Mass spectrometry (Tokyo, Japan) · 2013Article
- Lipidomic and spatio-temporal imaging of fat by mass spectrometry in mice duodenum during lipid digestion.PloS one · 2013Article
- Multivariate analysis of ToF-SIMS data from multicomponent systems: the why, when, and how.Biointerphases · 2012Review
- Lipid imaging with time-of-flight secondary ion mass spectrometry (ToF-SIMS).Biochimica et biophysica acta · 2011Review
- Plasma lipidomics reveals potential prognostic signatures within a cohort of cystic fibrosis patients.Journal of lipid research · 2011Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cftr knockout mouse model of cystic fibrosis (CF) shows intestinal obstruction; malabsorption and inflammation; and a fatty acid imbalance in intestinal mucosa. We performed a lipid mapping of colon sections from CF and control (WT) mice by cluster time of flight secondary-ion mass spectrometry (TOF-SIMS) imaging to localize lipid alterations. Data were processed either manually or by multivariate statistical methods. TOF-SIMS analysis showed a particular localization for cholesteryl sulfate at the epithelial border, C16:1 fatty acid in Lieberkühn glands, and C18:0 fatty acid in lamina propria and submucosa. Significant increases in vitamin E (vE) and C16:0 fatty acid in the epithelial border of CF colon were detected. Principal component analysis (PCA) and partitioning clustering allowed us to characterize different structural regions of colonic mucosa according to variations in C14:0, C16:0, C16:1, C18:0, C18:1, C18:2, C20:3, C20:4, and C22:6 fatty acids; phosphatidylethanolamine, phosphatidylcholine, and phosphatidylinositol glycerolipids; cholesterol; vitamin E; and cholesteryl sulfate. PCA on spectra from Lieberkühn glands led to separation of CF and WT individuals. This study shows for the first time the spatial distribution of lipids in colonic mucosa and suggests TOF-SIMS plus multivariate analyses as a powerful tool to investigate disease-related tissue spatial lipid signatures.
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