ArticleBlood2010
Spatiotemporal organization, regulation, and functions of tractions during neutrophil chemotaxis.
Article in Blood, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 47 citations in OpenAlex.
- The Protein Kinase aPKC as Well as the Small GTPases RhoA and Cdc42 Regulates Neutrophil Chemotaxis Partly by Recruiting the ROCK Kinase to the Leading Edge.Genes to cells : devoted to molecular & cellular mechanisms · 2025Article
- Article
- Mechanosensitive traction force generation is regulated by the neutrophil activation state.Scientific reports · 2023Article
- Additive Manufacturing of Viscoelastic Polyacrylamide Substrates for Mechanosensing Studies.ACS omega · 2022Article
- Article
- Leukocyte Cytoskeleton Polarization Is Initiated by Plasma Membrane Curvature from Cell Attachment.Developmental cell · 2019Article
- Rotation of stress fibers as a single wheel in migrating fish keratocytes.Scientific reports · 2018Article
- Fast-crawling cell types migrate to avoid the direction of periodic substratum stretching.Cell adhesion & migration · 2016Article
- Hybrid mechanosensing system to generate the polarity needed for migration in fish keratocytes.Cell adhesion & migration · 2016Article
- Blood and immune cell engineering: Cytoskeletal contractility and nuclear rheology impact cell lineage and localization: Biophysical regulation of hematopoietic differentiation and trafficking.BioEssays : news and reviews in molecular, cellular and developmental biology · 2015Review
- Moesin and myosin phosphatase confine neutrophil orientation in a chemotactic gradient.The Journal of experimental medicine · 2015Article
- Matrix confinement plays a pivotal role in regulating neutrophil-generated tractions, speed, and integrin utilization.The Journal of biological chemistry · 2015Article
- Cytoskeletal Mechanics Regulating Amoeboid Cell Locomotion.Applied mechanics reviews · 2014Article
- Both contractile axial and lateral traction force dynamics drive amoeboid cell motility.The Journal of cell biology · 2014Article
- Mammalian target of rapamycin and Rictor control neutrophil chemotaxis by regulating Rac/Cdc42 activity and the actin cytoskeleton.Molecular biology of the cell · 2013Article
- Mechanobiology of bone marrow stem cells: from myosin-II forces to compliance of matrix and nucleus in cell forms and fates.Differentiation; research in biological diversity · 2013Review
- A PLCβ/PI3Kγ-GSK3 signaling pathway regulates cofilin phosphatase slingshot2 and neutrophil polarization and chemotaxis.Developmental cell · 2011Article
- Measuring traction forces of motile dendritic cells on micropost arrays.Biophysical journal · 2011Article
- The non-receptor tyrosine kinase Lyn controls neutrophil adhesion by recruiting the CrkL-C3G complex and activating Rap1 at the leading edge.Journal of cell science · 2011Article
- Role of mechanical factors in fate decisions of stem cells.Regenerative medicine · 2011Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 3 institutions in 1 country.
Funding
Abstract
Despite recent advances in our understanding of biochemical regulation of neutrophil chemotaxis, little is known about how mechanical factors control neutrophils' persistent polarity and rapid motility. Here, using a human neutrophil-like cell line and human primary neutrophils, we describe a dynamic spatiotemporal pattern of tractions during chemotaxis. Tractions are located at both the leading and the trailing edge of neutrophils, where they oscillate with a defined periodicity. Interestingly, traction oscillations at the leading and the trailing edge are out of phase with the tractions at the front leading those at the back, suggesting a temporal mechanism that coordinates leading edge and trailing edge activities. The magnitude and periodicity of tractions depend on the activity of nonmuscle myosin IIA. Specifically, traction development at the leading edge requires myosin light chain kinase-mediated myosin II contractility and is necessary for α5β1-integrin activation and leading edge adhesion. Localized myosin II activation induced by spatially activated small GTPase Rho, and its downstream kinase p160-ROCK, as previously reported, leads to contraction of actin-myosin II complexes at the trailing edge, causing it to de-adhere. Our data identify a key biomechanical mechanism for persistent cell polarity and motility.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.