ArticleJournal of experimental botany2010
Duplicate polyphenol oxidase genes on barley chromosome 2H and their functional differentiation in the phenol reaction of spikes and grains.
Article in Journal of experimental botany, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 40 citations in OpenAlex.
- Birth and death evolution of polyphenol oxidase (PPO) gene family in Oryza species.Botanical studies · 2026Article
- Biochemical Analysis of Recombinant Pea Seed Coat-Specific Polyphenol Oxidase (Journal of agricultural and food chemistry · 2025Article
- Crop Evolution of Foxtail Millet.Plants (Basel, Switzerland) · 2024Review
- Genome-wide analysis of the polyphenol oxidase gene family reveals that MaPPO1 and MaPPO6 are the main contributors to fruit browning inFrontiers in plant science · 2023Article
- Polyphenol oxidase genes in barley (Frontiers in plant science · 2023Article
- Multi-target genome editing reduces polyphenol oxidase activity in wheat (Frontiers in plant science · 2023Article
- Chlorophyll deficiency delays but does not prevent melanogenesis in barley seed melanoplasts.Protoplasma · 2022Article
- Features of Activity of the Phenylpropanoid Biosynthesis Pathway in Melanin-Accumulating Barley Grains.Frontiers in plant science · 2022Article
- Article
- The R2R3 Transcription Factor CsMYB59 Regulates Polyphenol Oxidase GeneFrontiers in plant science · 2021Article
- Reduced Enzymatic Browning in Potato Tubers by Specific Editing of a Polyphenol Oxidase GeneFrontiers in plant science · 2019Article
- Genetic Mapping and Discovery of the Candidate Gene for Black Seed Coat Color in Watermelon (Frontiers in plant science · 2019Article
- Genomic Variation Among and Within SixG3 (Bethesda, Md.) · 2018Article
- Polyphenol Oxidases in Crops: Biochemical, Physiological and Genetic Aspects.International journal of molecular sciences · 2017Article
- A PPO Promoter from Betalain-Producing Red Swiss Chard, Directs Petiole- and Root-Preferential Expression of Foreign Gene in Anthocyanins-Producing Plants.International journal of molecular sciences · 2015Article
- Multiple origins of the phenol reaction negative phenotype in foxtail millet, Setaria italica (L.) P. Beauv., were caused by independent loss-of-function mutations of the polyphenol oxidase (Si7PPO) gene during domestication.Molecular genetics and genomics : MGG · 2015Article
- Marker-trait associations in Virginia Tech winter barley identified using genome-wide mapping.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2013Article
- Hordeum chilense genome, a useful tool to investigate the endosperm yellow pigment content in the Triticeae.BMC plant biology · 2012Article
- The polyphenol oxidase gene family in land plants: Lineage-specific duplication and expansion.BMC genomics · 2012Article
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Polyphenol oxidases (PPOs) are copper-containing metalloenzymes encoded in the nucleus and transported into the plastids. Reportedly, PPOs cause time-dependent discoloration (browning) of end-products of wheat and barley, which impairs their appearance quality. For this study, two barley PPO homologues were amplified using PCR with a primer pair designed in the copper binding domains of the wheat PPO genes. The full-lengths of the respective PPO genes were cloned using a BAC library, inverse-PCR, and 3'-RACE. Linkage analysis showed that the polymorphisms in PPO1 and PPO2 co-segregated with the phenol reaction phenotype of awns. Subsequent RT-PCR experiments showed that PPO1 was expressed in hulls and awns, and that PPO2 was expressed in the caryopses. Allelic variation of PPO1 and PPO2 was analysed in 51 barley accessions with the negative phenol reaction of awns. In PPO1, amino acid substitutions of five types affecting functionally important motif(s) or C-terminal region(s) were identified in 40 of the 51 accessions tested. In PPO2, only one mutant allele with a precocious stop codon resulting from an 8 bp insertion in the first exon was found in three of the 51 accessions tested. These observations demonstrate that PPO1 is the major determinant controlling the phenol reaction of awns. Comparisons of PPO1 single mutants and the PPO1PPO2 double mutant indicate that PPO2 controls the phenol reaction in the crease on the ventral side of caryopses. An insertion of a hAT-family transposon in the promoter region of PPO2 may be responsible for different expression patterns of the duplicate PPO genes in barley.
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