ArticleProceedings of the National Academy of Sciences of the United States of America2010
Global analysis of trans-splicing in Drosophila.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 81 papers.
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Who cites it
81 citing papers in PubMed, 106 citations in OpenAlex.
- Pervasive noise in human pre-mRNA splice site selection.Molecular cell · 2026Article
- Multiple intron motifs have redundant functions in the trans-splicing of the mod(mdg4) locus.Nucleic acids research · 2026Article
- The hidden sources of spurious fusion transcripts in plants.Genome biology · 2026Article
- Evaluating the reliability of tools for mRNA annotation and IRES studies.bioRxiv : the preprint server for biology · 2026Article
- Trans-Splicing Enhances Processivity of RNA Polymerase II and Suppresses Polyadenylation Signals.Doklady. Biochemistry and biophysics · 2026Article
- The "cutting edge" of non-canonical RNA splicing.Frontiers in molecular biosciences · 2026Review
- Promoter Specificity and Transcription Levels ModulateInternational journal of molecular sciences · 2025Article
- Chimeric RNA isoforms generated by diverse mechanisms from two C-type lectins modulate innate immunity in arthropods.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Article
- Investigation of the Functional Role of the Conserved Sequence at the 5'-End of the Fourth Intron of the mod(mdg4) Gene in Trans-Splicing in Drosophila melanogaster.Doklady. Biochemistry and biophysics · 2025Article
- An Investigation of the Conservatism of Sequences Defining trans-Splicing in the mod(mdg4) Locus across Drosophila and Silkworm Species.Doklady. Biochemistry and biophysics · 2025Article
- A Novel Mechanism for Transcription Termination in theBiology · 2024Article
- Co-transcriptional splicing facilitates transcription of gigantic genes.PLoS genetics · 2024Article
- Multiple strategies, including 6mA methylation, affecting plant alternative splicing in allopolyploid peanut.Plant biotechnology journal · 2024Article
- Detecting intragenic trans-splicing events from non-co-linearly spliced junctions by hybrid sequencing.Nucleic acids research · 2023Article
- Artifacts and biases of the reverse transcription reaction in RNA sequencing.RNA (New York, N.Y.) · 2023Review
- CRISPR/Cas9-based split homing gene drive targetingProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Assessing the impacts of various factors on circular RNA reliability.Life science alliance · 2023Article
- Trans-splicing facilitated by RNA pairing greatly expands sDscam isoform diversity but not homophilic binding specificity.Science advances · 2022Article
- Computational analysis of sense-antisense chimeric transcripts reveals their potential regulatory features and the landscape of expression in human cells.NAR genomics and bioinformatics · 2021Article
21 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Precursor mRNA (pre-mRNA) splicing can join exons contained on either a single pre-mRNA (cis) or on separate pre-mRNAs (trans). It is exceedingly rare to have trans-splicing between protein-coding exons and has been demonstrated for only two Drosophila genes: mod(mdg4) and lola. It has also been suggested that trans-splicing is a mechanism for the generation of chimeric RNA products containing sequence from multiple distant genomic sites. Because most high-throughput approaches cannot distinguish cis- and trans-splicing events, the extent to which trans-splicing occurs between protein-coding exons in any organism is unknown. Here, we used paired-end deep sequencing of mRNA to identify genes that undergo trans-splicing in Drosophila interspecies hybrids. We did not observe credible evidence for the existence of chimeric RNAs generated by trans-splicing of RNAs transcribed from distant genomic loci. Rather, our data suggest that experimental artifacts are the source of most, if not all, apparent chimeric RNA products. We did, however, identify 80 genes that appear to undergo trans-splicing between homologous alleles and can be classified into three categories based on their organization: (i) genes with multiple 3' terminal exons, (ii) genes with multiple first exons, and (iii) genes with very large introns, often containing other genes. Our results suggest that trans-splicing between homologous alleles occurs more commonly in Drosophila than previously believed and may facilitate expression of architecturally complex genes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.