ArticleBMC genomics2010
Recovery of the mitochondrial COI barcode region in diverse Hexapoda through tRNA-based primers.
Article in BMC genomics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- Population Genomics Approaches Identify a Cryptic, Emerging Generalist Pest Complex.Ecology and evolution · 2026Article
- Revised relationships of the endemic New Zealand subfamily Pronkiinae stat. nov. (Hymenoptera, Braconidae).ZooKeys · 2026Article
- Development of a Multiplex PCR Assay for Simultaneous Identification of Six Commercially Important Bivalves.Foods (Basel, Switzerland) · 2025Article
- Diversity and the Origin ofInsects · 2025Article
- Microsatellite and Mitochondrial COI Provide Novel Insights Into the Population Genetic Structure of White Prunicola Scale (Ecology and evolution · 2025Article
- First record of the genusZooKeys · 2025Article
- Pest categorisation ofEFSA journal. European Food Safety Authority · 2024Article
- Characterising the north-western European species ofBiodiversity data journal · 2024Article
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- Improving DNA barcoding library of armored scale insects (Hemiptera: Diaspididae) in China.PloS one · 2024Article
- Establishing reliable DNA barcoding primers for jumping plant lice (Psylloidea, Hemiptera).BMC research notes · 2023Article
- Two Species Delimitation ofInsects · 2023Article
- Article
- Complete mitochondrial genome ofParasitology · 2023Article
- Another crack in the Dark Taxa wall: a custom DNA barcoding protocol for the species-rich and common Eurytomidae (Hymenoptera, Chalcidoidea).Biodiversity data journal · 2023Article
- Two new genera and one new species of the tribe Adeshini (Hymenoptera, Braconidae, Braconinae) from India and South Africa.ZooKeys · 2023Article
- Identification of fish species through tRNA-based primer design.BMC bioinformatics · 2022Article
- Species Diversity and Phylogenetic Relationships of Olive Lace Bugs (Hemiptera: Tingidae) Found in South Africa.Insects · 2021Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDNA barcoding uses a 650 bp segment of the mitochondrial cytochrome c oxidase I (COI) gene as the basis for an identification system for members of the animal kingdom and some other groups of eukaryotes. PCR amplification of the barcode region is a key step in the analytical chain, but it sometimes fails because of a lack of homology between the standard primer sets and target DNA.
resultsTwo forward PCR primers were developed following analysis of all known arthropod mitochondrial genome arrangements and sequence alignment of the tRNA-W gene which was usually located within 200 bp upstream of the COI gene. These two primers were combined with a standard reverse primer (LepR1) to produce a cocktail which generated a barcode amplicon from 125 of 141 species that included representatives of 121 different families of Hexapoda. High quality sequences were recovered from 79% of the species including groups, such as scale insects, that invariably fail to amplify with standard primers.
conclusionsA cocktail of two tRNA-W forward primers coupled with a standard reverse primer amplifies COI for most hexapods, allowing characterization of the standard barcode primer binding region in COI 5' as well as the barcode segment. The current results show that primers designed to bind to highly conserved gene regions upstream of COI will aid the amplification of this gene region in species where standard primers fail and provide valuable information to design a primer for problem groups.
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