ArticleBMC genomics2010
Comparative analysis of secreted protein evolution using expressed sequence tags from four poplar leaf rusts (Melampsora spp.).
Article in BMC genomics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 62 citations in OpenAlex.
- Genome-wide prediction of secretory proteins in Ceratocystis paradoxa and expression analysis of effectors during early infection.BMC microbiology · 2026Article
- The Fungal Effector Mlp37347 Alters Plasmodesmata Fluxes and Enhances Susceptibility to Pathogen.Microorganisms · 2021Article
- Antivirulence and avirulence genes in human pathogenic fungi.Virulence · 2019Review
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- Positively Selected Effector Genes and Their Contribution to Virulence in the Smut Fungus Sporisorium reilianum.Genome biology and evolution · 2018Article
- Infection assays in Arabidopsis reveal candidate effectors from the poplar rust fungus that promote susceptibility to bacteria and oomycete pathogens.Molecular plant pathology · 2018Article
- The prediction of a pathogenesis-related secretome of Puccinia helianthi through high-throughput transcriptome analysis.BMC bioinformatics · 2017Article
- The Hemileia vastatrix effector HvEC-016 suppresses bacterial blight symptoms in coffee genotypes with the SThe New phytologist · 2017Article
- Effector-Mining in the Poplar Rust Fungus Melampsora larici-populina Secretome.Frontiers in plant science · 2015Review
- The effect on the transcriptome of Anemone coronaria following infection with rust (Tranzschelia discolor).PloS one · 2015Article
- High-resolution transcript profiling of the atypical biotrophic interaction between Theobroma cacao and the fungal pathogen Moniliophthora perniciosa.The Plant cell · 2014Article
- Compositions of fungal secretomes indicate a greater impact of phylogenetic history than lifestyle adaptation.BMC genomics · 2014Article
- The haustorial transcriptomes of Uromyces appendiculatus and Phakopsora pachyrhizi and their candidate effector families.Molecular plant pathology · 2014Article
- Using transcription of six Puccinia triticina races to identify the effective secretome during infection of wheat.Frontiers in plant science · 2014Article
- Duplications and losses in gene families of rust pathogens highlight putative effectors.Frontiers in plant science · 2014Article
- The genome sequence and effector complement of the flax rust pathogen Melampsora lini.Frontiers in plant science · 2014Article
- Patterns of genomic variation in the poplar rust fungus Melampsora larici-populina identify pathogenesis-related factors.Frontiers in plant science · 2014Article
- Annotation of a hybrid partial genome of the coffee rust (Hemileia vastatrix) contributes to the gene repertoire catalog of the Pucciniales.Frontiers in plant science · 2014Article
- Methodologies to decipher the cell secretome.Biochimica et biophysica acta · 2013Review
- Mosaic genome structure of the barley powdery mildew pathogen and conservation of transcriptional programs in divergent hosts.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundObligate biotrophs such as rust fungi are believed to establish long-term relationships by modulating plant defenses through a plethora of effector proteins, whose most recognizable feature is the presence of a signal peptide for secretion. Since the phenotypes of these effectors extend to host cells, their genes are expected to be under accelerated evolution stimulated by host-pathogen coevolutionary arms races. Recently, whole genome sequence data has allowed the prediction of secretomes, facilitating the identification of putative effectors.
resultsWe generated cDNA libraries from four poplar leaf rust pathogens (Melampsora spp.) and used computational approaches to identify and annotate putative secreted proteins with the aim of uncovering new knowledge about the nature and evolution of the rust secretome. While more than half of the predicted secretome members encoded lineage-specific proteins, similarities with experimentally characterized fungal effectors were also identified. A SAGE analysis indicated a strong stage-specific regulation of transcripts encoding secreted proteins. The average sequence identity of putative secreted proteins to their closest orthologs in the wheat stem rust Puccinia graminis f. sp. tritici was dramatically reduced compared with non-secreted ones. A comparative genomics approach based on homologous gene groups unravelled positive selection in putative members of the secretome.
conclusionWe uncovered robust evidence that different evolutionary constraints are acting on the rust secretome when compared to the rest of the genome. These results are consistent with the view that these genes are more likely to exhibit an effector activity and be involved in coevolutionary arms races with host factors.
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