ArticlePloS one2010
Identification of inappropriately reprogrammed genes by large-scale transcriptome analysis of individual cloned mouse blastocysts.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 40 citations in OpenAlex.
- Manipulating the Epigenome in Nuclear Transfer Cloning: Where, When and How.International journal of molecular sciences · 2020Review
- The effect of cell penetrating peptide-conjugated coactivator-associated arginine methyltransferase 1 (CPP-CARM1) on the cloned mouse embryonic development.Scientific reports · 2018Article
- KDM4B-mediated reduction of H3K9me3 and H3K36me3 levels improves somatic cell reprogramming into pluripotency.Scientific reports · 2017Article
- Reprogramming towards totipotency is greatly facilitated by synergistic effects of small molecules.Biology open · 2017Article
- Identification of key factors conquering developmental arrest of somatic cell cloned embryos by combining embryo biopsy and single-cell sequencing.Cell discovery · 2016Article
- Chromatin condensation of Xist genomic loci during oogenesis in mice.Development (Cambridge, England) · 2015Article
- Transcriptomic Features of Bovine Blastocysts Derived by Somatic Cell Nuclear Transfer.G3 (Bethesda, Md.) · 2015Article
- Discovery of porcine maternal factors related to nuclear reprogramming and early embryo development by proteomic analysis.Proteome science · 2015Article
- The role of maternal-specific H3K9me3 modification in establishing imprinted X-chromosome inactivation and embryogenesis in mice.Nature communications · 2014Article
- Totipotency: what it is and what it is not.Stem cells and development · 2014Article
- Identification and characterization of an oocyte factor required for porcine nuclear reprogramming.The Journal of biological chemistry · 2014Article
- Chromatin changes in reprogramming of mammalian somatic cells.Rejuvenation research · 2014Review
- The transcriptomic architecture of mouse Sertoli cell clone embryos reveals temporal–spatial-specific reprogramming.Reproduction (Cambridge, England) · 2013Article
- Recent advancements in cloning by somatic cell nuclear transfer.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2013Review
- Nuclear reprogramming of sperm and somatic nuclei in eggs and oocytes.Reproductive medicine and biology · 2013Article
- Somatic donor cell type correlates with embryonic, but not extra-embryonic, gene expression in postimplantation cloned embryos.PloS one · 2013Article
- Reprogramming and development in nuclear transfer embryos and in interspecific systems.Current opinion in genetics & development · 2012Review
- Comparative analysis of nuclear transfer embryo-derived mouse embryonic stem cells. Part II: gene regulation.Cellular reprogramming · 2012Article
- More with less Xist.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- X-linked gene transcription patterns in female and male in vivo, in vitro and cloned porcine individual blastocysts.PloS one · 2012Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although cloned embryos generated by somatic/embryonic stem cell nuclear transfer (SECNT) certainly give rise to viable individuals, they can often undergo embryonic arrest at any stage of embryogenesis, leading to diverse morphological abnormalities. In an effort to gain further insights into reprogramming and the properties of SECNT embryos, we performed a large-scale gene expression profiling of 87 single blastocysts using GeneChip microarrays. Sertoli cells, cumulus cells, and embryonic stem cells were used as donor cells. The gene expression profiles of 87 blastocysts were subjected to microarray analysis. Using principal component analysis and hierarchical clustering, the gene expression profiles were clearly classified into 3 clusters corresponding to the type of donor cell. The results revealed that each type of SECNT embryo had a unique gene expression profile that was strictly dependent upon the type of donor cells, although there was considerable variation among the individual profiles within each group. This suggests that the reprogramming process is distinct for embryos cloned from different types of donor cells. Furthermore, on the basis of the results of comparison analysis, we identified 35 genes that were inappropriately reprogrammed in most of the SECNT embryos; our findings demonstrated that some of these genes, such as Asz1, Xlr3a and App, were appropriately reprogrammed only in the embryos with a transcriptional profile that was the closest to that of the controls. Our findings provide a framework to further understand the reprogramming in SECNT embryos.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.