ArticlePloS one2010
Structural changes of the paraflagellar rod during flagellar beating in Trypanosoma cruzi.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 23 citations in OpenAlex.
- Plasma membrane invaginations and their diverse cellular functions.BMC biology · 2026Review
- Quantitative assessment of the nanoanatomy of the contractile vacuole complex inLife science alliance · 2024Article
- Contribution of microscopy to a better understanding of the anatomy of pathogenic protists.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Multiparametric Atomic Force Microscopy Identifies Multiple Structural and Physical Heterogeneities on the Surface ofACS omega · 2020Article
- Touching the Surface: Diverse Roles for the Flagellar Membrane in Kinetoplastid Parasites.Microbiology and molecular biology reviews : MMBR · 2020Review
- Antitrypanosomal Activities and Mechanisms of Action of Novel Tetracyclic Iridoids from Morinda lucida Benth.Antimicrobial agents and chemotherapy · 2016Article
- Simulating the complex cell design of Trypanosoma brucei and its motility.PLoS computational biology · 2015Article
- The eukaryotic flagellum makes the day: novel and unforeseen roles uncovered after post-genomics and proteomics data.Current protein & peptide science · 2012Review
- Interactive multimedia to teach the life cycle of Trypanosoma cruzi, the causative agent of Chagas disease.PLoS neglected tropical diseases · 2012Article
- Cryo-electron tomography and 3-D analysis of the intact flagellum in Trypanosoma brucei.Journal of structural biology · 2012Article
- Adhesion of Trypanosoma cruzi trypomastigotes to fibronectin or laminin modifies tubulin and paraflagellar rod protein phosphorylation.PloS one · 2012Article
- Three-dimensional structure of the Trypanosome flagellum suggests that the paraflagellar rod functions as a biomechanical spring.PloS one · 2012Article
- Short-term follow-up of chagasic patients after benzonidazole treatment using multiple serological markers.BMC infectious diseases · 2011Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTrypanosoma cruzi, the agent of Chagas disease, is a protozoan member of the Kinetoplastidae family characterized for the presence of specific and unique structures that are involved in different cell activities. One of them is the paraflagellar rod (PFR), a complex array of filaments connected to the flagellar axoneme. Although the function played by the PFR is not well established, it has been shown that silencing of the synthesis of its major proteins by either knockout of RNAi impairs and/or modifies the flagellar motility. METHODOLOGY/PRINCIPAL
findingsHere, we present results obtained by atomic force microscopy (AFM) and transmission electron microscopy (TEM) of replicas of quick-frozen, freeze-fractured, deep-etched and rotary-replicated cells to obtain detailed information of the PFR structures in regions of the flagellum in straight and in bent state. The images obtained show that the PFR is not a fixed and static structure. The pattern of organization of the PFR filament network differs between regions of the flagellum in a straight state and those in a bent state. Measurements of the distances between the PFR filaments and the filaments that connect the PFR to the axoneme as well as of the angles between the intercrossed filaments supported this idea. CONCLUSIONS/SIGNIFICANCE: Graphic computation based on the information obtained allowed the proposal of an animated model for the PFR structure during flagellar beating and provided a new way of observing PFR filaments during flagellar beating.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.