ArticlePLoS computational biology2010
The 20S proteasome splicing activity discovered by SpliceMet.
Article in PLoS computational biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 65 citations in OpenAlex.
- Protein degradation by human 20S proteasomes elucidates the interplay between peptide hydrolysis and splicing.Nature communications · 2024Article
- T-Cell Receptor Repertoire Sequencing in the Era of Cancer Immunotherapy.Clinical cancer research : an official journal of the American Association for Cancer Research · 2023Article
- InvitroSPI and a large database of proteasome-generated spliced and non-spliced peptides.Scientific data · 2023Article
- Sources of Cancer Neoantigens beyond Single-Nucleotide Variants.International journal of molecular sciences · 2022Review
- Predicting the Success of Fmoc-Based Peptide Synthesis.ACS omega · 2022Article
- Article
- Neo-Splicetopes in Tumor Therapy: A Lost Case?Frontiers in immunology · 2022Review
- RHybridFinder: An R package to process immunopeptidomic data for putative hybrid peptide discovery.STAR protocols · 2021Article
- Spliced HLA-bound peptides: a Black Swan event in immunology.Clinical and experimental immunology · 2021Review
- In vitro proteasome processing of neo-splicetopes does not predict their presentation in vivo.eLife · 2021Article
- Commentary: Are There Indeed Spliced Peptides in the Immunopeptidome?Molecular & cellular proteomics : MCP · 2021Article
- Response: Commentary: AnFrontiers in immunology · 2021Article
- Are There Indeed Spliced Peptides in the Immunopeptidome?Molecular & cellular proteomics : MCP · 2021Article
- Large database for the analysis and prediction of spliced and non-spliced peptide generation by proteasomes.Scientific data · 2020Article
- Antigen processing and presentation in HIV infection.Molecular immunology · 2019Review
- Proteolytic dynamics of human 20S thymoproteasome.The Journal of biological chemistry · 2019Article
- Global Identification of Post-Translationally Spliced Peptides with Neo-Fusion.Journal of proteome research · 2019Article
- AnFrontiers in immunology · 2019Article
- Mapping the MHC Class I-Spliced Immunopeptidome of Cancer Cells.Cancer immunology research · 2019Article
- Islet stress, degradation and autoimmunity.Diabetes, obesity & metabolism · 2018Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The identification of proteasome-generated spliced peptides (PSP) revealed a new unpredicted activity of the major cellular protease. However, so far characterization of PSP was entirely dependent on the availability of patient-derived cytotoxic CD8+ T lymphocytes (CTL) thus preventing a systematic investigation of proteasome-catalyzed peptide splicing (PCPS). For an unrestricted PSP identification we here developed SpliceMet, combining the computer-based algorithm ProteaJ with in vitro proteasomal degradation assays and mass spectrometry. By applying SpliceMet for the analysis of proteasomal processing products of four different substrate polypeptides, derived from human tumor as well as viral antigens, we identified fifteen new spliced peptides generated by PCPS either by cis or from two separate substrate molecules, i.e., by trans splicing. Our data suggest that 20S proteasomes represent a molecular machine that, due to its catalytic and structural properties, facilitates the generation of spliced peptides, thereby providing a pool of qualitatively new peptides from which functionally relevant products may be selected.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.