ArticleThe ISME journal2011
Single-cell genomics reveals the lifestyle of Poribacteria, a candidate phylum symbiotically associated with marine sponges.
Article in The ISME journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 123 papers, 1 of them a synthesis that pooled it.
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Who cites it
123 citing papers in PubMed, 1 synthesis or guideline pooled it, 278 citations in OpenAlex.
- Planctomycetes as Host-Associated Bacteria: A Perspective That Holds Promise for Their Future Isolations, by Mimicking Their Native Environmental Niches in Clinical Microbiology Laboratories.Frontiers in cellular and infection microbiology · 2020Pooled it
- New insights into the microbiome of the deep-sea spongeMicrobial genomics · 2026Article
- Functional Genomic Characteristics of Marine Sponge-AssociatedMicroorganisms · 2025Article
- Unveiling the significance of prokaryotic composition from ferromanganese crusts regarding the interlink between cobalt and vitamin BFrontiers in microbiology · 2025Article
- Chengkuizengella axinellae sp. nov., a symbiotic bacterium isolated from a marine sponge of the genus Axinella.Antonie van Leeuwenhoek · 2024Article
- High microbiome and metabolome diversification in coexisting sponges with different bio-ecological traits.Communications biology · 2024Article
- Diversity and ecological function of urease-producing bacteria in the cultivation environment of Gracilariopsis lemaneiformis.Microbial ecology · 2024Article
- Taurine as a key intermediate for host-symbiont interaction in the tropical sponge Ianthella basta.The ISME journal · 2023Article
- Synthase-selected sorting approach identifies a beta-lactone synthase in a nudibranch symbiotic bacterium.Microbiome · 2023Article
- Research progress of single-cell sequencing in tuberculosis.Frontiers in immunology · 2023Review
- Microbiome diversity and metabolic capacity determines the trophic ecology of the holobiont in Caribbean sponges.ISME communications · 2022Article
- Opportunities and challenges of using metagenomic data to bring uncultured microbes into cultivation.Microbiome · 2022Article
- METABOLIC: high-throughput profiling of microbial genomes for functional traits, metabolism, biogeochemistry, and community-scale functional networks.Microbiome · 2022Article
- Beyond Soil-Dwelling Actinobacteria: Fantastic Antibiotics and Where to Find Them.Antibiotics (Basel, Switzerland) · 2022Review
- The natural product biosynthesis potential of the microbiomes of Earth - Bioprospecting for novel anti-microbial agents in the meta-omics era.Computational and structural biotechnology journal · 2022Review
- Single-cell genomics-based analysis reveals a vital ecological role ofMicrobial genomics · 2021Article
- Genomics and phylogeny of the proposed phylum 'Candidatus Poribacteria' associated with the excavating sponge Thoosa mismalolli.Antonie van Leeuwenhoek · 2021Article
- Comparative Genomics Provides Insight into the Function of Broad-Host Range Sponge Symbionts.mBio · 2021Article
- DNA-stable isotope probing (DNA-SIP) identifies marine sponge-associated bacteria actively utilizing dissolved organic matter (DOM).Environmental microbiology · 2021Article
- Genome Reduction and Secondary Metabolism of the Marine Sponge-Associated CyanobacteriumMarine drugs · 2021Article
63 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study, we present a single-cell genomics approach for the functional characterization of the candidate phylum Poribacteria, members of which are nearly exclusively found in marine sponges. The microbial consortia of the Mediterranean sponge Aplysina aerophoba were singularized by fluorescence-activated cell sorting, and individual microbial cells were subjected to phi29 polymerase-mediated 'whole-genome amplification'. Pyrosequencing of a single amplified genome (SAG) derived from a member of the Poribacteria resulted in nearly 1.6 Mb of genomic information distributed among 554 contigs analyzed in this study. Approximately two-third of the poribacterial genome was sequenced. Our findings shed light on the functional properties and lifestyle of a possibly ancient bacterial symbiont of marine sponges. The Poribacteria are mixotrophic bacteria with autotrophic CO(2)-fixation capacities through the Wood-Ljungdahl pathway. The cell wall is of Gram-negative origin. The Poribacteria produce at least two polyketide synthases (PKSs), one of which is the sponge-specific Sup-type PKS. Several putative symbiosis factors such as adhesins (bacterial Ig-like domains, lamininin G domain proteins), adhesin-related proteins (ankyrin, fibronectin type III) and tetratrico peptide repeat domain-encoding proteins were identified, which might be involved in mediating sponge-microbe interactions. The discovery of genes coding for 24-isopropyl steroids implies that certain fossil biomarkers used to date the origins of metazoan life on earth may possibly be of poribacterial origin. Single-cell genomic approaches, such as those shown herein, contribute to a better understanding of beneficial microbial consortia, of which most members are, because of the lack of cultivation, inaccessible by conventional techniques.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.