ArticleJournal of clinical microbiology2010
Multicentric comparative analytical performance study for molecular detection of low amounts of Toxoplasma gondii from simulated specimens.
Article in Journal of clinical microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 81 citations in OpenAlex.
- Multicenter performance evaluation of the "quanty TOXO (RH region)" kit (Clonit) for molecular diagnosis of toxoplasmosis.Journal of clinical microbiology · 2025Article
- Will we miss specific IgA detection for the diagnosis of congenital toxoplasmosis? A French retrospective monocenter study on 483 infants.Journal of clinical microbiology · 2025Article
- Performance evaluation of nine reference centers and comparison of DNA extraction protocols for effective surveillance of Leishmania-infected Phlebotomine sand flies: Basis for technical recommendations.PLoS neglected tropical diseases · 2024Article
- Trend in serological and molecular diagnostic methods for Toxoplasma gondii infection.European journal of medical research · 2024Review
- Molecular diagnosis of human toxoplasmosis: the state of the art.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2024Review
- Multicenter evaluation of theJournal of clinical microbiology · 2024Article
- Ocular toxoplasmosis: a review of the current diagnostic and therapeutic approaches.International ophthalmology · 2022Review
- Impact of pre-analytic step duration on molecular diagnosis of toxoplasmosis for five types of biological samples.PloS one · 2021Article
- Is Real-Time PCR Targeting Rep 529 Suitable for Diagnosis of Toxoplasmosis in Patients Infected with Non-Type II Strains in North America?Journal of clinical microbiology · 2020Article
- Multicentric Evaluation of the Bio-Evolution Toxoplasma gondii Assay for the Detection ofJournal of clinical microbiology · 2020Article
- Comparison of an in house and a commercial real-time polymerase chain reaction targeting Toxoplasma gondii RE gene using various samples collected from patients in Turkey.BMC infectious diseases · 2019Article
- Semi-Quantitative, Duplexed qPCR Assay for the Detection ofTropical medicine and infectious disease · 2019Article
- Comparison of PCR assays to detect Toxoplasma gondii oocysts in green-lipped mussels (Perna canaliculus).Parasitology research · 2019Article
- Performance of three molecular methods for detection ofFood and waterborne parasitology · 2019Article
- Development of a Novel Protocol Based on Blood Clot to Improve the Sensitivity of qPCR Detection ofThe American journal of tropical medicine and hygiene · 2019Article
- Detection of diverseWellcome open research · 2019Article
- Analytical Performance of a Loop-Mediated Isothermal Amplification Assay forThe American journal of tropical medicine and hygiene · 2018Article
- Evaluation of Toxoplasma ELITe MGB Real-Time PCR Assay for Diagnosis of Toxoplasmosis.Journal of clinical microbiology · 2017Article
- Analytical Performance of Four Polymerase Chain Reaction (PCR) and Real Time PCR (qPCR) Assays for the Detection of SixFrontiers in microbiology · 2017Article
- Performance Testing of PCR Assay in Blood Samples for the Diagnosis of Toxoplasmic Encephalitis in AIDS Patients from the French Departments of America and Genetic Diversity of Toxoplasma gondii: A Prospective and Multicentric Study.PLoS neglected tropical diseases · 2016Article
Corrections and comments
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Authors and funding
11 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although screening for maternal toxoplasmic seroconversion during pregnancy is based on immunodiagnostic assays, the diagnosis of clinically relevant toxoplasmosis greatly relies upon molecular methods. A problem is that this molecular diagnosis is subject to variation of performances, mainly due to a large diversity of PCR methods and primers and the lack of standardization. The present multicentric prospective study, involving eight laboratories proficient in the molecular prenatal diagnosis of toxoplasmosis, was a first step toward the harmonization of this diagnosis among university hospitals in France. Its aim was to compare the analytical performances of different PCR protocols used for Toxoplasma detection. Each center extracted the same concentrated Toxoplasma gondii suspension and tested serial dilutions of the DNA using its own assays. Differences in analytical sensitivities were observed between assays, particularly at low parasite concentrations (<or=2 T. gondii genomes per reaction tube), with "performance scores" differing by a 20-fold factor among laboratories. Our data stress the fact that differences do exist in the performances of molecular assays in spite of expertise in the matter; we propose that laboratories work toward a detection threshold defined for a best sensitivity of this diagnosis. Moreover, on the one hand, intralaboratory comparisons confirmed previous studies showing that rep529 is a more adequate DNA target for this diagnosis than the widely used B1 gene. But, on the other hand, interlaboratory comparisons showed differences that appear independent of the target, primers, or technology and that hence rely essentially on proficiency and care in the optimization of PCR conditions.
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