ArticleMolecular biology of the cell2010
Chloride intracellular channel 4 is critical for the epithelial morphogenesis of RPE cells and retinal attachment.
Article in Molecular biology of the cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 43 citations in OpenAlex.
- Review of the clinical electrooculogram - Part 1: Mechanism of the Light-Rise.Documenta ophthalmologica. Advances in ophthalmology · 2026Review
- Human retinal organoid model of disease-relevant photoreceptor cell death amenable to drug screening.Cell death & disease · 2026Article
- The development of a methodology to measure primary ciliary length in neural progenitor cells with a target molecule knocked down.Biochemistry and biophysics reports · 2026Article
- Clic6 Deficiency Triggers Aberrant Apical Microvilli in RPE.Investigative ophthalmology & visual science · 2025Article
- Ion channels research in hPSC-RPE cells: bridging benchwork to clinical applications.Journal of translational medicine · 2024Review
- The retinal pigment epithelium: Functions and roles in ocular diseases.Fundamental research · 2024Review
- Differential Effect of Aldosterone or Mineralocorticoid Receptor Overexpression on Retinal Inflammation.Investigative ophthalmology & visual science · 2024Article
- Visual pigment-deficient cones survive and mediate visual signaling despite the lack of outer segments.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Retinal pigment epithelium-specific CLIC4 mutant is a mouse model of dry age-related macular degeneration.Nature communications · 2022Article
- Characterization and allogeneic transplantation of a novel transgenic cone-rich donor mouse line.Experimental eye research · 2021Article
- A Literature-Derived Knowledge Graph Augments the Interpretation of Single Cell RNA-seq Datasets.Genes · 2021Article
- Altered photoreceptor metabolism in mouse causes late stage age-related macular degeneration-like pathologies.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Article
- CLIC4 regulates late endosomal trafficking and matrix degradation activity of MMP14 at focal adhesions in RPE cells.Scientific reports · 2019Article
- Gene-Edited Human Kidney Organoids Reveal Mechanisms of Disease in Podocyte Development.Stem cells (Dayton, Ohio) · 2017Article
- The Biology of Ciliary Dynamics.Cold Spring Harbor perspectives in biology · 2017Review
- CLIC1 regulates dendritic cell antigen processing and presentation by modulating phagosome acidification and proteolysis.Biology open · 2016Article
- CLIC4 regulates apical exocytosis and renal tube luminogenesis through retromer- and actin-mediated endocytic trafficking.Nature communications · 2016Article
- Sex differences in the acute in vivo effects of different human SP-A variants on the mouse alveolar macrophage proteome.Journal of proteomics · 2014Article
- Detection of differential fetal and adult expression of chloride intracellular channel 4 (CLIC4) protein by analysis of a green fluorescent protein knock-in mouse line.BMC developmental biology · 2014Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Retinal detachment is a sight-threatening condition. The molecular mechanism underlying the adhesion between the RPE and photoreceptors is poorly understood because the intimate interactions between these two cell types are impossible to model and study in vitro. In this article, we show that chloride intracellular channel 4 (CLIC4) is enriched at apical RPE microvilli, which are interdigitated with the photoreceptor outer segment. We used a novel plasmid-based transfection method to cell-autonomously suppress CLIC4 in RPE in situ. CLIC4 silenced RPE cells exhibited a significant loss of apical microvilli and basal infoldings, reduced retinal adhesion, and epithelial-mesenchymal transition. Ectopically expressing ezrin failed to rescue the morphological changes exerted by CLIC4 silencing. Neural retinas adjacent to the CLIC4-suppressed RPE cells display severe dysplasia. Finally, a high level of aquaporin 1 unexpectedly appeared at the apical surfaces of CLIC4-suppressed RPE cells, together with a concomitant loss of basal surface expression of monocarboxylate transporter MCT3. Our results suggested that CLIC4 plays an important role in RPE-photoreceptor adhesion, perhaps by modulating the activity of cell surface channels/transporters. We propose that these changes may be attributable to subretinal fluid accumulation in our novel retinal detachment animal model.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.