ArticleRNA (New York, N.Y.)2010
Retention of spliceosomal components along ligated exons ensures efficient removal of multiple introns.
Article in RNA (New York, N.Y.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Elimination of myotonia improves myopathy in a muscleblind-like knockout model of myotonic dystrophy.Nature communications · 2026Article
- Impact of U2-type introns on splice site prediction in A. thaliana species using deep learning.BMC bioinformatics · 2025Article
- Emerging and re-emerging themes in co-transcriptional pre-mRNA splicing.Molecular cell · 2024Review
- Selection of Olduvai Domains during Evolution: A Role for Primate-Specific Splicing Super-Enhancer and RNA Guanine Quadruplex in BipartiteBrain sciences · 2022Article
- Long-read sequencing of nascent RNA reveals coupling among RNA processing events.Genome research · 2018Article
- Widespread intra-dependencies in the removal of introns from human transcripts.Nucleic acids research · 2017Article
- Knockdown and replacement therapy mediated by artificial mirtrons in spinocerebellar ataxia 7.Nucleic acids research · 2017Article
- The exon junction complex as a node of post-transcriptional networks.Nature reviews. Molecular cell biology · 2016Review
- Reverse transcriptase and intron number evolution.Stem cell investigation · 2014Article
- Transcriptome-wide modulation of splicing by the exon junction complex.Genome biology · 2014Article
- The nuclear cap-binding complex interacts with the U4/U6·U5 tri-snRNP and promotes spliceosome assembly in mammalian cells.RNA (New York, N.Y.) · 2013Article
- Identifying the mechanisms of intron gain: progress and trends.Biology direct · 2012Review
- Deep intron elements mediate nested splicing events at consecutive AG dinucleotides to regulate alternative 3' splice site choice in vertebrate 4.1 genes.Molecular and cellular biology · 2012Article
- Specific Y14 domains mediate its nucleo-cytoplasmic shuttling and association with spliced mRNA.Scientific reports · 2011Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The majority of mammalian pre-mRNAs contains multiple introns that are excised prior to export and translation. After intron excision, ligated exon intermediates participate in subsequent intron excisions. However, exon ligation generates an exon of increased size, a feature of pre-mRNA splicing that can interfere with downstream splicing events. These considerations raise the question of whether unique mechanisms exist that permit efficient removal of introns neighboring ligated exons. Kinetic analyses of multiple intron-containing pre-mRNAs revealed that splicing is more efficient following an initial intron removal event, suggesting that either the recruitment of the exon junction complex (EJC) to ligated exons increases the efficiency of multiple intron excisions or that the initial definition of splice sites is sufficient to permit efficient splicing of introns neighboring ligated exons. Knockdown experiments show that the deposition of the EJC does not affect subsequent splicing kinetics. Instead, spliceosomal components that are not involved in the initial splicing event remain associated with the pre-mRNA to ensure efficient removal of neighboring introns. Thus, ligated exons do not require redefinition, providing an additional kinetic advantage for exon defined splice sites.
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