ArticleMolecular endocrinology (Baltimore, Md.)2010
Luteinizing hormone receptor-stimulated progesterone production by preovulatory granulosa cells requires protein kinase A-dependent activation/dephosphorylation of the actin dynamizing protein cofilin.
Article in Molecular endocrinology (Baltimore, Md.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 49 citations in OpenAlex.
- Actomyosin contraction in the follicular epithelium provides the major mechanical force for follicle rupture duringProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Role of thyroid stimulating hormone in the maintenance and functioning of the human corpus luteum.Journal of endocrinological investigation · 2024Article
- Luteinizing hormone stimulates ingression of mural granulosa cells within the mouse preovulatory follicle†.Biology of reproduction · 2024Article
- Phosphatases modified by LH signaling in ovarian follicles: testing their role in regulating the NPR2 guanylyl cyclase†.Biology of reproduction · 2024Article
- Phosphatases modified by LH signaling in ovarian follicles: testing their role in regulating the NPR2 guanylyl cyclase.bioRxiv : the preprint server for biology · 2023Article
- Cofilin activation in pancreatic acinar cells plays a pivotal convergent role for mediating CCK-stimulated enzyme secretion and growth.Frontiers in physiology · 2023Article
- Luteinizing Hormone Regulation of Inter-Organelle Communication and Fate of the Corpus Luteum.International journal of molecular sciences · 2021Review
- Mechanistic insight into how gonadotropin hormone receptor complexes direct signaling†.Biology of reproduction · 2020Review
- Transcriptome analysis during photostimulated recrudescence reveals distinct patterns of gene regulation in Siberian hamster ovaries†.Biology of reproduction · 2020Article
- Integrative Proteomic and Phosphoproteomic Analyses of Granulosa Cells During Follicular Atresia in Porcine.Frontiers in cell and developmental biology · 2020Article
- Proteomic Analysis of Porcine Pre-ovulatory Follicle Differentiation Into Corpus Luteum.Frontiers in endocrinology · 2019Article
- Reelin and aromatase cooperate in ovarian follicle development.Scientific reports · 2018Article
- The role of combining medroxyprogesterone 17-acetate with human menopausal gonadotropin in mouse ovarian follicular development.Scientific reports · 2018Article
- GATA Regulation and Function During the Ovarian Life Cycle.Vitamins and hormones · 2018Review
- Article
- Forkhead box O member FOXO1 regulates the majority of follicle-stimulating hormone responsive genes in ovarian granulosa cells.Molecular and cellular endocrinology · 2016Article
- Dephosphorylation of MAP2D enhances its binding to vimentin in preovulatory ovarian granulosa cells.Journal of cell science · 2016Article
- Pre-translational regulation of luteinizing hormone receptor in follicular somatic cells of cattle.Animal reproduction science · 2015Article
- Expression and activity of Rac1 is negatively affected in the dehydroepiandrosterone induced polycystic ovary of mouse.Journal of ovarian research · 2014Article
- GATA4 and GATA6 silencing in ovarian granulosa cells affects levels of mRNAs involved in steroidogenesis, extracellular structure organization, IGF-I activity, and apoptosis.Endocrinology · 2013Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Activation of the LH receptor (LHR) on preovulatory granulosa cells stimulates the cAMP/protein kinase A (PKA) pathway to regulate expression of genes required for ovulation and luteinization. LHR signaling also initiates rearrangement of the actin cytoskeleton. Because disruption of the actin cytoskeleton has been causally linked to steroidogenesis in various cell models, we sought to identify the cellular mechanisms that may modulate reorganization of the actin cytoskeleton and to determine whether cytoskeletal reorganization is required for steroidogenesis. Herein we report that LHR signaling in preovulatory granulosa cells promotes rapid dephosphorylation of the actin-depolymerizing factor cofilin at Ser3 that is dependent on PKA. The LHR-stimulated dephosphorylation of cofilin(Ser3) switches on cofilin activity to bind actin filaments and enhance their dynamics. Basal phosphorylation of cofilin(Ser3) is mediated by active/GTP-bound Rho and downstream protein kinases; LHR signaling promotes a decrease in active/GTP-bound Rho by a PKA-dependent mechanism. LHR-dependent Rho inactivation and subsequent activation of cofilin does not involve ERK, epidermal growth factor receptor, or phosphatidylinositol 3-kinase pathways downstream of PKA. To understand the biological significance of cofilin activation, preovulatory granulosa cells were transduced with a mutant cofilin adenoviral vector in which Ser3 was mutated to Glu (S-E cofilin). Inactive S-E cofilin abolished LHR-mediated reorganization of the actin cytoskeleton and caused a 70% decrease in LHR-stimulated progesterone that is obligatory for ovulation. Taken together, these results show that LHR signaling via PKA activates a cofilin-regulated rearrangement of the actin cytoskeleton and that active cofilin is required to initiate progesterone secretion by preovulatory granulosa cells.
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