ArticleNucleic acids research2010
Facilitated leaky scanning and atypical ribosome shunting direct downstream translation initiation on the tricistronic S1 mRNA of avian reovirus.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 20 citations in OpenAlex.
- Uncovering the Underlying Mechanisms Blocking Replication of Bluetongue Virus Serotype 26 (BTV-26) inBiomolecules · 2023Article
- The nonstructural p17 protein of a fusogenic bat-borne reovirus regulates viral replication in virus species- and host-specific manners.PLoS pathogens · 2022Article
- Evidence for a non-fusogenic aquareovirus encoding a transmembrane protein.Archives of virology · 2022Article
- Ribosomal control in RNA virus-infected cells.Frontiers in microbiology · 2022Review
- Noncanonical Translation Initiation in Eukaryotes.Cold Spring Harbor perspectives in biology · 2019Review
- Cell-cell fusion induced by reovirus FAST proteins enhances replication and pathogenicity of non-enveloped dsRNA viruses.PLoS pathogens · 2019Article
- Unusual dicistronic expression from closely spaced initiation codons in an umbravirus subgenomic RNA.Nucleic acids research · 2018Article
- Synthesis and Translation of Viral mRNA in Reovirus-Infected Cells: Progress and Remaining Questions.Viruses · 2018Review
- Ribosomal frameshifting and transcriptional slippage: From genetic steganography and cryptography to adventitious use.Nucleic acids research · 2016Review
- Death of a dogma: eukaryotic mRNAs can code for more than one protein.Nucleic acids research · 2016Review
- Viral and Cellular mRNA Translation in Coronavirus-Infected Cells.Advances in virus research · 2016Review
- Tuning gene expression with synthetic upstream open reading frames.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Dual short upstream open reading frames control translation of a herpesviral polycistronic mRNA.PLoS pathogens · 2013Article
- Non-canonical translation in RNA viruses.The Journal of general virology · 2012Review
- Animal virus schemes for translation dominance.Current opinion in virology · 2011Review
- Virion structure of baboon reovirus, a fusogenic orthoreovirus that lacks an adhesion fiber.Journal of virology · 2011Article
- Detection of a fourth orbivirus non-structural protein.PloS one · 2011Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The S1 mRNA of avian reovirus is functionally tricistronic, encoding three unrelated proteins, p10, p17 and σC, from three sequential, partially overlapping open reading frames (ORFs). The mechanism of translation initiation at the 3'-proximal σC ORF is currently unknown. Transient RNA transfections using Renilla luciferase reporter constructs revealed only a modest reduction in reporter expression upon optimization of either the p10 or p17 start sites. Insertion of multiple upstream AUG (uAUG) codons in a preferred start codon sequence context resulted in a substantial retention of downstream translation initiation on the S1 mRNA, but not on a heterologous mRNA. The S1 mRNA therefore facilitates leaky scanning to promote ribosome access to the σC start codon. Evidence also indicates that σC translation is mediated by a second scanning-independent mechanism capable of bypassing upstream ORFs. This alternate mechanism is cap-dependent and requires a sequence-dependent translation enhancer element that is complementary to 18S rRNA. Downstream translation initiation of the tricistronic S1 mRNA is therefore made possible by two alternate mechanisms, facilitated leaky scanning and an atypical form of ribosome shunting. This dual mechanism of downstream translation initiation ensures sufficient expression of the σC cell attachment protein that is essential for infectious progeny virus production.
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