ArticleThe Journal of biological chemistry2010
Localization of myosin 1b to actin protrusions requires phosphoinositide binding.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed.
- The unconventional regulation of non-muscle myosins.Biophysical reviews · 2026Article
- Myosin Class I Genes Follow a Cell Type-Specific Transcription Pattern in Human Haematopoietic Cell Lines.International journal of molecular sciences · 2026Article
- MYO1B in human disease: an actin-based motor linking membrane trafficking to oncogenic signaling, metastasis, and vascular aging.Frontiers in oncology · 2026Review
- Myo1b Promotes Premature Endothelial Senescence and Dysfunction via Suppressing Autophagy: Implications for Vascular Aging.Oxidative medicine and cellular longevity · 2023Article
- Class I Myosins, molecular motors involved in cell migration and cancer.Cell adhesion & migration · 2022Article
- In pancreatic β-cells myosin 1b regulates glucose-stimulated insulin secretion by modulating an early step in insulin granule trafficking from the Golgi.Molecular biology of the cell · 2021Article
- A cryo-electron tomography workflow reveals protrusion-mediated shedding on injured plasma membrane.Science advances · 2021Article
- Myosin 1b promotes migration, invasion and glycolysis in cervical cancer via ERK/HIF-1α pathway.American journal of translational research · 2021Article
- Basic-hydrophobic sites are localized in conserved positions inside and outside of PH domains and affect localization ofMolecular biology of the cell · 2020Article
- Recent advances in PTEN signalling axes in cancer.Faculty reviews · 2020Review
- Unconventional Myosins: How Regulation Meets Function.International journal of molecular sciences · 2019Review
- Myosin 1b is an actin depolymerase.Nature communications · 2019Article
- Myosin 1b Regulates Nuclear AKT Activation by Preventing Localization of PTEN in the Nucleus.iScience · 2019Article
- Myosin 1b promotes axon formation by regulating actin wave propagation and growth cone dynamics.The Journal of cell biology · 2018Article
- Arginase-II activates mTORC1 through myosin-1b in vascular cell senescence and apoptosis.Cell death & disease · 2018Article
- Passenger strand of miR-145-3p acts as a tumor-suppressor by targeting MYO1B in head and neck squamous cell carcinoma.International journal of oncology · 2018Article
- Myosin 1b and F-actin are involved in the control of secretory granule biogenesis.Scientific reports · 2017Article
- Knockdown of Unconventional Myosin ID Expression Induced Morphological Change in Oligodendrocytes.ASN neuro · 2016Article
- Myosin-I molecular motors at a glance.Journal of cell science · 2016Review
- Force Generation by Membrane-Associated Myosin-I.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Myosin 1b (Myo1b), a class I myosin, is a widely expressed, single-headed, actin-associated molecular motor. Transient kinetic and single-molecule studies indicate that it is kinetically slow and responds to tension. Localization and subcellular fractionation studies indicate that Myo1b associates with the plasma membrane and certain subcellular organelles such as endosomes and lysosomes. Whether Myo1b directly associates with membranes is unknown. We demonstrate here that full-length rat Myo1b binds specifically and with high affinity to phosphatidylinositol 4,5-bisphosphate (PIP(2)) and phosphatidylinositol 3,4,5-triphosphate (PIP(3)), two phosphoinositides that play important roles in cell signaling. Binding is not Ca(2+)-dependent and does not involve the calmodulin-binding IQ region in the neck domain of Myo1b. Furthermore, the binding site is contained entirely within the C-terminal tail region, which contains a putative pleckstrin homology domain. Single mutations in the putative pleckstrin homology domain abolish binding of the tail domain of Myo1b to PIP(2) and PIP(3) in vitro. These same mutations alter the distribution of Myc-tagged Myo1b at membrane protrusions in HeLa cells where PIP(2) localizes. In addition, we found that motor activity is required for Myo1b localization in filopodia. These results suggest that binding of Myo1b to phosphoinositides plays an important role in vivo by regulating localization to actin-enriched membrane projections.
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