ArticleBiomaterials2010
Modular enzymatically crosslinked protein polymer hydrogels for in situ gelation.
Article in Biomaterials, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 104 citations in OpenAlex.
- Smart responsive hydrogels combined with AI design for tumor therapy.Acta pharmaceutica Sinica. B · 2026Review
- Genetic and bioactive functionalization of bioinks for 3D bioprinting.Bioprocess and biosystems engineering · 2025Review
- From Synthesis to Solutions: Hydrogels' Impact on the Biomedical Landscape.Current pharmaceutical biotechnology · 2025Review
- Biomedical Application of Enzymatically Crosslinked Injectable Hydrogels.Gels (Basel, Switzerland) · 2024Review
- Applications of self-assembled peptide hydrogels in anti-tumor therapy.Nanoscale advances · 2024Review
- Hydrogels for Cardiac Restorative Support: Relevance of Gelation Mechanisms for Prospective Clinical Use.Current heart failure reports · 2023Review
- Recombinant mucin biotechnology and engineering.Advanced drug delivery reviews · 2023Review
- Versatility of Hydrogels: From Synthetic Strategies, Classification, and Properties to Biomedical Applications.Gels (Basel, Switzerland) · 2022Review
- Recent advancements in enzyme-mediated crosslinkable hydrogels:APL bioengineering · 2021Review
- Engineering High-Yield Biopolymer Secretion Creates an Extracellular Protein Matrix for Living Materials.mSystems · 2021Article
- Microrheology for biomaterial design.APL bioengineering · 2020Review
- Silk Hydrogels Crosslinked by the Fenton Reaction.Advanced healthcare materials · 2019Article
- Thermosensitive, fast gelling, photoluminescent, highly flexible, and degradable hydrogels for stem cell delivery.Acta biomaterialia · 2019Article
- Cross-Linked Hydrogel for Pharmaceutical Applications: A Review.Advanced pharmaceutical bulletin · 2017Review
- Atelocollagen-based Hydrogels Crosslinked with Oxidised Polysaccharides as Cell Encapsulation Matrix for Engineered Bioactive Stromal Tissue.Tissue engineering and regenerative medicine · 2017Article
- Cell-laden microfluidic microgels for tissue regeneration.Lab on a chip · 2016Review
- Combinatorial codon scrambling enables scalable gene synthesis and amplification of repetitive proteins.Nature materials · 2016Article
- Bioactive factor delivery strategies from engineered polymer hydrogels for therapeutic medicine.Progress in polymer science · 2014Article
- Designing ECM-mimetic materials using protein engineering.Acta biomaterialia · 2014Review
- Controlling topological entanglement in engineered protein hydrogels with a variety of thiol coupling chemistries.Frontiers in chemistry · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Biomaterials that mimic the extracellular matrix in both modularity and crosslinking chemistry have the potential to recapitulate the instructive signals that ultimately control cell fate. Toward this goal, modular protein polymer-based hydrogels were created through genetic engineering and enzymatic crosslinking. Animal derived tissue transglutaminase (tTG) and recombinant human transglutaminase (hTG) enzymes were used for coupling two classes of protein polymers containing either lysine or glutamine, which have the recognition substrates for enzymatic crosslinking evenly spaced along the protein backbone. Utilizing tTG under physiological conditions, complete crosslinking occurred within 2 min, as determined by particle tracking microrheology. Hydrogel composition impacted the elastic storage modulus of the gel over 4-fold and also influenced microstructure and degree of swelling, but did not appreciably effect degradation by plasmin. Mouse 3T3 and primary human fibroblasts were cultured in both 2- and 3-dimensions without a decrease in cell viability and displayed spreading in 2D. The properties, which are controlled through the specific nature of the protein polymer precursors, render these gels valuable for in situ therapies. Furthermore, the modular hydrogel composition allows tailoring of mechanical and physical properties for specific tissue engineering applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.