ArticleJournal of cancer research and clinical oncology2011
Specific protein and miRNA patterns characterise tumour-associated fibroblasts in bladder cancer.
Article in Journal of cancer research and clinical oncology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 47 citations in OpenAlex.
- Fundamental and Clinical Applications of Materials Based on Cancer-Associated Fibroblasts in Cancers.International journal of molecular sciences · 2021Review
- MiR-20a-5p Negatively Regulates NR4A3 to Promote Metastasis in Bladder Cancer.Journal of oncology · 2021Article
- Review
- Review
- Biological heterogeneity and versatility of cancer-associated fibroblasts in the tumor microenvironment.Oncogene · 2019Review
- Epigenetics of Bladder Cancer: Where Biomarkers and Therapeutic Targets Meet.Frontiers in genetics · 2019Review
- Epigenetic Crosstalk between the Tumor Microenvironment and Ovarian Cancer Cells: A Therapeutic Road Less Traveled.Cancers · 2018Review
- The microRNA-15a-PAI-2 axis in cholangiocarcinoma-associated fibroblasts promotes migration of cancer cells.Molecular cancer · 2018Article
- MiR-411 suppresses the development of bladder cancer by regulating ZnT1.OncoTargets and therapy · 2018Article
- Cancer associated fibroblasts: An essential role in the tumor microenvironment.Oncology letters · 2017Article
- Genetic alterations and epigenetic alterations of cancer-associated fibroblasts.Oncology letters · 2017Article
- miR-31 affects colorectal cancer cells by inhibiting autophagy in cancer-associated fibroblasts.Oncotarget · 2016Article
- Epithelial plasticity in urothelial carcinoma: Current advancements and future challenges.World journal of stem cells · 2016Review
- [Epigenetic regulation of urological tumors. Importance for prognosis and metastasis].Der Urologe. Ausg. A · 2015Review
- Epigenetic silencing of microRNA-149 in cancer-associated fibroblasts mediates prostaglandin E2/interleukin-6 signaling in the tumor microenvironment.Cell research · 2015Article
- [Tumour-stroma interactions in urothelial cancer].Der Urologe. Ausg. A · 2015Review
- Immunological basis in the pathogenesis and treatment of bladder cancer.Expert review of clinical immunology · 2015Review
- Involvement of the Androgen and Glucocorticoid Receptors in Bladder Cancer.International journal of endocrinology · 2015Review
- In vivo delivery of miRNAs for cancer therapy: challenges and strategies.Advanced drug delivery reviews · 2015Review
- Identification of biomarkers for radiation-induced acute intestinal symptoms (RIAISs) in cervical cancer patients by serum protein profiling.Journal of radiation research · 2015Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTumour development and progression are strongly affected by interaction of tumour cells and tumour stroma. Different tumour models demonstrate a supportive effect of tumour-associated fibroblasts (TAF) on the tumour genesis. Aims of the present study are the isolation of TAF from primary urinary bladder tumour specimens and the proteomic and epigenetic characterisation.
methodsTAF were isolated from cultured urinary bladder tumour specimens. Therefore, primary tumour material was treated with EDTA followed by two separated detachment steps. Non-tumour fibroblasts were isolated from foreskin and normal bladder tissues. Proteins and total RNA were isolated from cultured fibroblasts. Protein pattern analyses were carried out by SELDI-TOF-MS. The miRNA expression profile was analysed by miRNA microarray.
resultsBy optimising cell culture routines, we achieved to isolate and subsequently cultivate TAF from primary tumour material of the urinary bladder. SELDI-TOF-MS measurements reveal distinct differences in the proteomic patterns of TAF and non-tumour fibroblasts. Microarray analyses indicate specific expression of several miRNAs in TAF and non-tumour fibroblasts.
conclusionIn summary, we determined proteomic and epigenetic differences between non-tumour fibroblasts and TAF of urinary bladder carcinoma and identified specific protein expression patterns as well as miRNA profiles of TAF in comparison with non-tumour fibroblasts. These findings provide more insights into the complex tumour network and a good starting point for the identification of markers for the prediction of tumour development and progression based on specific TAF expression patterns.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.