ArticleInfection and immunity2010
Activation of the CpxRA system by deletion of cpxA impairs the ability of Haemophilus ducreyi to infect humans.
Article in Infection and immunity, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
44 citing papers in PubMed, 60 citations in OpenAlex.
- Phenotypic Effects of a Small Molecule CpxA Phosphatase Inhibitor on Uropathogenic Escherichia coli.ACS infectious diseases · 2026Article
- Regulation of the Yersinia pseudotuberculosis Type III Secretion System by the CpxAR Two-Component System.Molecular microbiology · 2026Article
- SFV Replicon Vector Harbouring Porcine Epidemic Diarrhoea Virus Immunogens Delivered by AttenuatedViruses · 2026Article
- Anti-infective compounds forSustainable microbiology · 2026Review
- Molecular pathogenesis ofMicrobiology and molecular biology reviews : MMBR · 2025Review
- Regulatory role of the Cpx ESR in bacterial behaviours.Virulence · 2024Review
- Cpx-signalling innpj antimicrobials and resistance · 2024Article
- Article
- The Two-Component System CpxRA Represses Salmonella Pathogenicity Island 2 by Directly Acting on theMicrobiology spectrum · 2022Article
- CpxA Phosphatase Inhibitor Activates CpxRA and Is a Potential Treatment for Uropathogenic Escherichia coli in a Murine Model of Infection.Microbiology spectrum · 2022Article
- Cpx-signalling facilitates Hms-dependent biofilm formation by Yersinia pseudotuberculosis.NPJ biofilms and microbiomes · 2022Article
- Comparative Genomic Analysis of the DUF34 Protein Family Suggests Role as a Metal Ion Chaperone or Insertase.Biomolecules · 2021Article
- Article
- Interactions of the Skin PathogenFrontiers in immunology · 2020Review
- Improved Prodigiosin Production by Relieving CpxR Temperature-Sensitive Inhibition.Frontiers in bioengineering and biotechnology · 2020Article
- First-generation structure-activity relationship studies of 2,3,4,9-tetrahydro-1H-carbazol-1-amines as CpxA phosphatase inhibitors.Bioorganic & medicinal chemistry letters · 2019Article
- Article
- Evaluation of CpxRA as a Therapeutic Target for Uropathogenic Escherichia coli Infections.Infection and immunity · 2018Article
- The Virulence Effect of CpxRA inFrontiers in cellular and infection microbiology · 2018Article
- Both MisR (CpxR) and MisS (CpxA) Are Required for Neisseria gonorrhoeae Infection in a Murine Model of Lower Genital Tract Infection.Infection and immunity · 2017Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
Haemophilus ducreyi must adapt to the environment of the human host to establish and maintain infection in the skin. Bacteria generally utilize stress response systems, such as the CpxRA two-component system, to adapt to hostile environments. CpxRA is the only obvious two-component system contained in the H. ducreyi genome and negatively regulates the lspB-lspA2 operon, which encodes proteins that enable the organism to resist phagocytosis. We constructed an unmarked, in-frame H. ducreyi cpxA deletion mutant, 35000HPDeltacpxA. In human inoculation experiments, 35000HPDeltacpxA formed papules at a rate and size that were significantly less than its parent and was unable to form pustules compared to the parent. CpxA usually has kinase and phosphatase activities for CpxR, and the deletion of CpxA leads to the accumulation of activated CpxR due to the loss of phosphatase activity and the ability of CpxR to accept phosphate groups from other donors. Using a reporter construct, the lspB-lspA2 promoter was downregulated in 35000HPDeltacpxA, confirming that CpxR was activated. Deletion of cpxA downregulated DsrA, the major determinant of serum resistance in the organism, causing the mutant to become serum susceptible. Complementation in trans restored parental phenotypes. 35000HPDeltacpxA is the first H. ducreyi mutant that is impaired in its ability to form both papules and pustules in humans. Since a major function of CpxRA is to control the flow of protein traffic across the periplasm, uncontrolled activation of this system likely causes dysregulated expression of multiple virulence determinants and cripples the ability of the organism to adapt to the host.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.