ArticlePlant physiology2010
Virus-induced gene silencing of plastidial soluble inorganic pyrophosphatase impairs essential leaf anabolic pathways and reduces drought stress tolerance in Nicotiana benthamiana.
Article in Plant physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 74 citations in OpenAlex.
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- Transcriptomics and starch biosynthesis analysis in leaves and developing seeds of mung bean provide a basis for genetic engineering of starch composition and seed quality.Frontiers in plant science · 2024Article
- Identifying loci controlling total starch content of leaf in Nicotiana tabacum through genome-wide association study.Functional & integrative genomics · 2022Article
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- Crystal Structure of Inorganic Pyrophosphatase FromFrontiers in cell and developmental biology · 2021Article
- Transcriptome analysis of drought-tolerant sorghum genotype SC56 in response to water stress reveals an oxidative stress defense strategy.Molecular biology reports · 2020Article
- Profiling of advanced glycation end products uncovers abiotic stress-specific target proteins in Arabidopsis.Journal of experimental botany · 2019Article
- Mutagenesis Reveals That theFrontiers in plant science · 2019Article
- Vacuolar Proton Pyrophosphatase Is Required for High Magnesium Tolerance inInternational journal of molecular sciences · 2018Article
- Vacuolar HThe Plant cell · 2018Article
- Inhibition of plastid PPase and NTT leads to major changes in starch and tuber formation in potato.Journal of experimental botany · 2018Article
- Metabolomics and proteomics reveal drought-stress responses of leaf tissues from spring-wheat.Scientific reports · 2018Article
- UDP-Sugar Producing Pyrophosphorylases: Distinct and Essential Enzymes With Overlapping Substrate Specificities, ProvidingFrontiers in plant science · 2018Review
- Comparative transcriptomics reveals a reduction in carbon capture and flux between source and sink in cytokinin-treated inflorescences of3 Biotech · 2018Article
- ThPP1 gene, encodes an inorganic pyrophosphatase in Thellungiella halophila, enhanced the tolerance of the transgenic rice to alkali stress.Plant cell reports · 2017Article
- Identification of Phosphorylation Sites Altering Pollen Soluble Inorganic Pyrophosphatase Activity.Plant physiology · 2017Article
- Molecular responses of genetically modified maize to abiotic stresses as determined through proteomic and metabolomic analyses.PloS one · 2017Article
- Vacuum and Co-cultivation Agroinfiltration of (Germinated) Seeds Results in Tobacco Rattle Virus (TRV) Mediated Whole-Plant Virus-Induced Gene Silencing (VIGS) in Wheat and Maize.Frontiers in plant science · 2017Article
- Analysis of the Role of the Drought-Induced GeneFrontiers in plant science · 2017Article
- Comparative proteomic analysis reveals the positive effect of exogenous spermidine on photosynthesis and salinity tolerance in cucumber seedlings.Plant cell reports · 2016Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The role of pyrophosphate in primary metabolism is poorly understood. Here, we report on the transient down-regulation of plastid-targeted soluble inorganic pyrophosphatase in Nicotiana benthamiana source leaves. Physiological and metabolic perturbations were particularly evident in chloroplastic central metabolism, which is reliant on fast and efficient pyrophosphate dissipation. Plants lacking plastidial soluble inorganic pyrophosphatase (psPPase) were characterized by increased pyrophosphate levels, decreased starch content, and alterations in chlorophyll and carotenoid biosynthesis, while constituents like amino acids (except for histidine, serine, and tryptophan) and soluble sugars and organic acids (except for malate and citrate) remained invariable from the control. Furthermore, translation of Rubisco was significantly affected, as observed for the amounts of the respective subunits as well as total soluble protein content. These changes were concurrent with the fact that plants with reduced psPPase were unable to assimilate carbon to the same extent as the controls. Furthermore, plants with lowered psPPase exposed to mild drought stress showed a moderate wilting phenotype and reduced vitality, which could be correlated to reduced abscisic acid levels limiting stomatal closure. Taken together, the results suggest that plastidial pyrophosphate dissipation through psPPase is indispensable for vital plant processes.
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