ArticleThe Journal of biological chemistry2010
A minimal cysteine motif required to activate the SKOR K+ channel of Arabidopsis by the reactive oxygen species H2O2.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 128 citations in OpenAlex.
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- Functional analysis of PagERF021 gene in salt stress tolerance in Populus alba × P. glandulosa.The plant genome · 2024Article
- Types of Membrane Transporters and the Mechanisms of Interaction between Them and Reactive Oxygen Species in Plants.Antioxidants (Basel, Switzerland) · 2024Review
- Reactive oxygen species- and nitric oxide-dependent regulation of ion and metal homeostasis in plants.Journal of experimental botany · 2023Article
- How Extracellular Reactive Oxygen Species Reach Their Intracellular Targets in Plants.Molecules and cells · 2023Review
- Membrane Proteins in Plant Salinity Stress Perception, Sensing, and Response.The Journal of membrane biology · 2023Review
- The RNA landscape ofFrontiers in plant science · 2023Article
- The captivating role of calcium in plant-microbe interaction.Frontiers in plant science · 2023Review
- Reactive oxygen species signalling in plant stress responses.Nature reviews. Molecular cell biology · 2022Review
- 12-Hydroxyjasmonic acid glucoside causes leaf-folding of Samanea saman through ROS accumulation.Scientific reports · 2022Article
- 2D material-based peroxidase-mimicking nanozymes: catalytic mechanisms and bioapplications.Analytical and bioanalytical chemistry · 2022Review
- Integrative transcriptomic and metabolomic analysis of D-leaf of seven pineapple varieties differing in N-P-K% contents.BMC plant biology · 2021Article
- Differential regulation of drought stress by biological membrane transporters and channels.Plant cell reports · 2021Review
- Hypoxia-induced increase in GABA content is essential for restoration of membrane potential and preventing ROS-induced disturbance to ion homeostasis.Plant communications · 2021Article
- The fast and the furious: rapid long-range signaling in plants.Plant physiology · 2021Review
- Modulation of Ion Transport Across Plant Membranes by Polyamines: Understanding Specific Modes of Action Under Stress.Frontiers in plant science · 2020Article
- The Ability to Regulate Transmembrane Potassium Transport in Root Is Critical for Drought Tolerance in Barley.International journal of molecular sciences · 2019Article
- Linking ploidy level with salinity tolerance: NADPH-dependent 'ROS-Ca2+ hub' in the spotlight.Journal of experimental botany · 2019Article
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Authors and funding
10 authors at 4 institutions in 4 countries.
Funding
Abstract
Reactive oxygen species (ROS) are essential for development and stress signaling in plants. They contribute to plant defense against pathogens, regulate stomatal transpiration, and influence nutrient uptake and partitioning. Although both Ca(2+) and K(+) channels of plants are known to be affected, virtually nothing is known of the targets for ROS at a molecular level. Here we report that a single cysteine (Cys) residue within the Kv-like SKOR K(+) channel of Arabidopsis thaliana is essential for channel sensitivity to the ROS H(2)O(2). We show that H(2)O(2) rapidly enhanced current amplitude and activation kinetics of heterologously expressed SKOR, and the effects were reversed by the reducing agent dithiothreitol (DTT). Both H(2)O(2) and DTT were active at the outer face of the membrane and current enhancement was strongly dependent on membrane depolarization, consistent with a H(2)O(2)-sensitive site on the SKOR protein that is exposed to the outside when the channel is in the open conformation. Cys substitutions identified a single residue, Cys(168) located within the S3 α-helix of the voltage sensor complex, to be essential for sensitivity to H(2)O(2). The same Cys residue was a primary determinant for current block by covalent Cys S-methioylation with aqueous methanethiosulfonates. These, and additional data identify Cys(168) as a critical target for H(2)O(2), and implicate ROS-mediated control of the K(+) channel in regulating mineral nutrient partitioning within the plant.
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