ArticleOphthalmology2010
Doxycycline's effect on ocular angiogenesis: an in vivo analysis.
Article in Ophthalmology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 51 citations in OpenAlex.
- Oral doxycycline reduces pterygium lesions; results from a double blind, randomized, placebo controlled clinical trial.PloS one · 2012Trial
- Doxycycline inhibits MMP-2 retinal activity and modulates the angiogenic processFrontiers in cell and developmental biology · 2025Article
- Key Clinical and Histopathological Features of a Pterygium-Like Induced Lesion in a Rabbit Model.Translational vision science & technology · 2024Article
- Minocycline and Diacetyl Minocycline Eye Drops Reduce Ocular Neovascularization in Mice.Translational vision science & technology · 2023Article
- Doxycycline Attenuated Ethanol-Induced Inflammaging in Endothelial Cells: Implications in Alcohol-Mediated Vascular Diseases.Antioxidants (Basel, Switzerland) · 2022Article
- Angiogenesis in Chronic Inflammatory Skin Disorders.International journal of molecular sciences · 2021Review
- Matrix Metalloproteinases in Age-Related Macular Degeneration (AMD).International journal of molecular sciences · 2020Review
- Identification and Interaction Analysis of Significant Genes and MicroRNAs in Pterygium.BioMed research international · 2019Article
- Tetracycline repurposing in neurodegeneration: focus on Parkinson's disease.Journal of neural transmission (Vienna, Austria : 1996) · 2018Review
- Extracellular matrix and fibroblast injection produces pterygium-like lesion in rabbits.Biological research · 2018Article
- Modern Therapeutic Approaches for Noninfectious Ocular Diseases Involving Inflammation.Advanced healthcare materials · 2017Review
- Ocular penetration of topically applied 1% tigecycline in a rabbit model.International journal of ophthalmology · 2017Article
- Oral Doxycycline Reduces the Total Number of Intraocular Bevacizumab Injections Needed to Control Neovascular Age-related Macular Degeneration.Medical hypothesis, discovery & innovation ophthalmology journal · 2017Article
- Myeloid-Specific Blockade of Notch Signaling Attenuates Choroidal Neovascularization through Compromised Macrophage Infiltration and Polarization in Mice.Scientific reports · 2016Article
- PGC-1α induces SPP1 to activate macrophages and orchestrate functional angiogenesis in skeletal muscle.Circulation research · 2014Article
- Effect of porcine chondrocyte-derived extracellular matrix on the pterygium in mouse model.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2014Article
- Doxycycline inhibits polarization of macrophages to the proangiogenic M2-type and subsequent neovascularization.The Journal of biological chemistry · 2014Article
- Synthesis and antibacterial activity of doxycycline neoglycosides.Journal of natural products · 2013Article
- Doxycycline prevents matrix remodeling and contraction by trichiasis-derived conjunctival fibroblasts.Investigative ophthalmology & visual science · 2013Article
- Silibinin, dexamethasone, and doxycycline as potential therapeutic agents for treating vesicant-inflicted ocular injuries.Toxicology and applied pharmacology · 2012Article
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Authors and funding
12 authors at 5 institutions in 1 country.
Funding
Abstract
purposeTo determine the in vivo effect of doxycycline on choroidal angiogenesis and pterygium growth by using a choroidal neovascular (CNV) murine model, a directed in vivo angiogenesis assay (DIVAA) and a pterygium murine model.
designExperimental study.
participantsThree murine models were investigated with 4 mice minimum per group and 22 maximum per group.
methodsMice received water with or without doxycycline. For the CNV, the neovascular lesion volume was determined in choroid-retinal pigment epithelial flat mounts using confocal microscopy 7 days after laser induction. For DIVAA, silicone capsules containing 10,000 human pterygium epithelial cells were implanted in the flanks of mice subcutaneously. After 11 days, neovascularization (NV) was quantified using spectrofluorometry after murine tail-vein injection of fluorescein isothiocyanate-labeled dextran. A pterygium epithelial cell model was developed by injecting 10,000 human pterygium epithelial cells in the nasal subconjunctival space in athymic nude mice. Doxycycline was started on day 6 at 50 mg/kg per day; corneal lesions that resulted from the injections were compared at days 6 and 15.
main outcome measuresThe Student t-test was used to evaluate the data for the CNV and DIVAA models and histologic preparations were used to evaluate pterygia lesions.
resultsThere was significantly less NV and lesion volume with doxycycline taken in drinking water versus plain water. With doxycycline treatment, the laser-induced CNV showed a maximal 66% decrease in choroidal blood vessel volume (P< or =0.008) and the DIVAA showed a 30% reduction of blood vessel growth and migration (P<0.004). Histologic preparations demonstrated that pterygium cell lesions regressed when mice were administered doxycycline for 9 days.
conclusionsDoxycycline significantly inhibited angiogenesis in 3 murine models. The most dramatic effect was found in the CNV model followed by the pterygia epithelial cell DIVAA model. The anterior segment pterygium model also showed regression histologically. This suggests that doxycycline may be successful as an adjunctive treatment for CNV and pterygia in humans; clinical trials would be necessary to determine if there is a benefit.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.