ArticleElectrophoresis2010
Microelectrophoresis platform for fast serial analysis of single cells.
Article in Electrophoresis, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed.
- G protein-coupled receptor kinase 3 modulates mesenchymal stem cell proliferation and differentiation through sphingosine-1-phosphate receptor regulation.Stem cell research & therapy · 2022Article
- Design of an automated capillary electrophoresis platform for single-cell analysis.Methods in enzymology · 2019Review
- Model acupuncture point: Bone marrow-derived stromal stem cells are moved by a weak electromagnetic field.World journal of stem cells · 2016Article
- Analysis of sphingosine kinase activity in single natural killer cells from peripheral blood.Integrative biology : quantitative biosciences from nano to macro · 2015Article
- An integrated microfluidic device for monitoring changes in nitric oxide production in single T-lymphocyte (Jurkat) cells.Analytical chemistry · 2013Article
- Microfluidic chemical cytometry of peptide degradation in single drug-treated acute myeloid leukemia cells.Analytical chemistry · 2013Article
- Automated capillary electrophoresis system for fast single-cell analysis.Analytical chemistry · 2013Article
- Article
- A hyphenated optical trap capillary electrophoresis laser induced native fluorescence system for single-cell chemical analysis.The Analyst · 2012Article
- Sampling techniques for single-cell electrophoresis.The Analyst · 2012Article
- Chemical analysis of single cells.Analytical chemistry · 2011Review
- Measuring enzyme activity in single cells.Trends in biotechnology · 2011Review
- Single-cell assays.Biomicrofluidics · 2011Article
- Continuous analysis of dye-loaded, single cells on a microfluidic chip.Lab on a chip · 2011Article
- Profiling metabolites and peptides in single cells.Nature methods · 2011Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
A capillary-based microelectrophoresis platform for fast serial analysis of single cells is described. In this system, the capillary remains fixed and a two-channel flow system is used to rapidly switch the buffer surrounding the capillary inlet from a physiological buffer to an electrophoretic buffer. Single cells are retained in the physiologic buffer channel utilizing an array of cell microwells patterned into the channel floor. The defined addresses of the cells on the array enable the sequential delivery of individual cells to the inlet of the capillary, where a focused laser pulse lyses the cell. The cell chamber is moved along a preordained route so that the inlet of the capillary is located in the electrophoresis buffer for separation and the physiological buffer during cell sampling. The throughput of the current system is limited by peak overlap between successive samples. Key characterizations of this system including the fluid flow rates, the cell array dimensions, and laser energies were performed. To demonstrate this system, 28 cells loaded with Oregon green and fluorescein were serially analyzed in under 16 min, a rate of 1.8 cells/min.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.