ArticleThe Journal of physiology2010
Replacing the rod with the cone transducin subunit decreases sensitivity and accelerates response decay.
Article in The Journal of physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 40 citations in OpenAlex.
- Article
- Analysis of dim-light responses in rod and cone photoreceptors with altered calcium kinetics.Journal of mathematical biology · 2023Article
- Mathematical analysis of phototransduction reaction parameters in rods and cones.Scientific reports · 2022Article
- Article
- Light responses of mammalian cones.Pflugers Archiv : European journal of physiology · 2021Review
- Analysis of waveform and amplitude of mouse rod and cone flash responses.The Journal of physiology · 2021Article
- A kinetic analysis of mouse rod and cone photoreceptor responses.The Journal of physiology · 2020Article
- Determination of basal phosphodiesterase activity in mouse rod photoreceptors with cGMP clamp.Scientific reports · 2019Article
- Regulation of calcium homeostasis in the outer segments of rod and cone photoreceptors.Progress in retinal and eye research · 2018Review
- Investigating the CaScientific reports · 2018Article
- Implications of dimeric activation of PDE6 for rod phototransduction.Open biology · 2018Article
- Light adaptation and the evolution of vertebrate photoreceptors.The Journal of physiology · 2017Article
- The evolution of rod photoreceptors.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Review
- The origin and evolution of cell types.Nature reviews. Genetics · 2016Review
- Why are rods more sensitive than cones?The Journal of physiology · 2016Review
- Quantitative modeling of the molecular steps underlying shut-off of rhodopsin activity in rod phototransduction.Molecular vision · 2016Article
- Exchange of Cone for Rod Phosphodiesterase 6 Catalytic Subunits in Rod Photoreceptors Mimics in Part Features of Light Adaptation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Modulation of mouse rod photoreceptor responses by Grb14 protein.The Journal of biological chemistry · 2014Article
- Molecular evolutionary analysis of vertebrate transducins: a role for amino acid variation in photoreceptor deactivation.Journal of molecular evolution · 2013Article
- Cone phosphodiesterase-6α' restores rod function and confers distinct physiological properties in the rod phosphodiesterase-6β-deficient rd10 mouse.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Cone vision is less sensitive than rod vision. Much of this difference can be attributed to the photoreceptors themselves, but the reason why the cones are less sensitive is still unknown. Recent recordings indicate that one important factor may be a difference in the rate of activation of cone transduction; that is, the rising phase of the cone response per bleached rhodopsin molecule (Rh*) has a smaller slope than the rising phase of the rod response per Rh*, perhaps because some step between Rh* and activation of the phosphodiesterase 6 (PDE6) effector molecule occurs with less gain. Since rods and cones have different G-protein alpha subunits, and since this subunit (Talpha) plays a key role both in the interaction of G-protein with Rh* and the activation of PDE6, we investigated the mechanism of the amplification difference by expressing cone Talpha in rod Talpha-knockout rods to produce so-called GNAT2C mice. We show that rods in GNAT2C mice have decreased sensitivity and a rate of activation half that of wild-type (WT) mouse rods. Furthermore, GNAT2C responses recover more rapidly than WT responses with kinetic parameters resembling those of native mouse cones. Our results show for the first time that part of the difference in sensitivity and response kinetics between rods and cones may be the result of a difference in the G-protein alpha subunit. They also indicate more generally that the molecular nature of G-protein alpha may play an important role in the kinetics of G-protein cascades for metabotropic receptors throughout the body.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.