ArticleMolecular cell2010
Hyperaccurate and error-prone ribosomes exploit distinct mechanisms during tRNA selection.
Article in Molecular cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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Who cites it
66 citing papers in PubMed, 111 citations in OpenAlex.
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- Many will enter, few will win: Cost and sensitivity of exploratory dynamics.Biophysical journal · 2026Article
- Mistranslation from an endogenous tRNA variant in human pan-genome cell lines.Nucleic acids research · 2026Article
- Collision-induced ribosome degradation driven by ribosome competition and translational perturbations.Nature communications · 2025Article
- Selective silencing of antibiotic-tethered ribosomes as a resistance mechanism against aminoglycosides.Nature communications · 2025Article
- Idiosyncratic evolvability among single-point ribosomal mutants towards multi-aminoglycoside resistance.PLoS genetics · 2025Article
- Translational error in mice increases with ageing in an organ-dependent manner.Nature communications · 2025Article
- An evolutionarily conserved phosphoserine-arginine salt bridge in the interface between ribosomal proteins uS4 and uS5 regulates translational accuracy in Saccharomyces cerevisiae.Nucleic acids research · 2024Article
- Continuous Fluorescence Assay for In Vitro Translation Compatible with Noncanonical Amino Acids.ACS synthetic biology · 2024Article
- Extensive breaking of genetic code degeneracy with non-canonical amino acids.Nature communications · 2023Article
- Bridging ribosomal synthesis to cell growth through the lens of kinetics.Biophysical journal · 2023Article
- Hyperaccurate Ribosomes for Improved Genetic Code Reprogramming.ACS synthetic biology · 2022Article
- Premature aging in mice with error-prone protein synthesis.Science advances · 2022Article
- The energy cost and optimal design of networks for biological discrimination.Journal of the Royal Society, Interface · 2022Article
- Increased fidelity of protein synthesis extends lifespan.Cell metabolism · 2021Article
- Silencing of the ER and Integrative Stress Responses in the Liver of Mice with Error-Prone Translation.Cells · 2021Article
- Directed evolution of rRNA improves translation kinetics and recombinant protein yield.Nature communications · 2021Article
- Random errors in protein synthesis activate an age-dependent program of muscle atrophy in mice.Communications biology · 2021Article
6 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Escherichia coli strains displaying hyperaccurate (restrictive) and ribosomal ambiguity (ram) phenotypes have long been associated with alterations in rpsL and rpsD/rpsE, respectively. Crystallographic evidence shows the ribosomal proteins S12 and S4/S5 (corresponding to these genes) to be located in separate regions of the small ribosomal subunit that are important for domain closure thought to take place during tRNA selection. Mechanistically, the process of tRNA selection is separated into two distinct phases, initial selection and proofreading. Here, we explore the effects of mutations in rpsL and rpsD on these steps. Surprisingly, both restrictive and ram ribosomes primarily affect tRNA selection through alteration of the off rates of the near-cognate tRNA species but during distinct phases of the overall process (proofreading and initial selection, respectively). These observations suggest that closure interfaces (S12/h27/h44 versus S4/S5) in two distinct regions of the small ribosomal subunit function independently to promote high-fidelity tRNA selection.
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