ArticleCritical care (London, England)2010
Time course of nitric oxide synthases, nitrosative stress, and poly(ADP ribosylation) in an ovine sepsis model.
Article in Critical care (London, England), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 45 citations in OpenAlex.
- Trial
- Effects of Normobaric Hypoxia and Adrenergic Blockade over 72 h on Cardiac Function in Rats.International journal of molecular sciences · 2023Article
- Fatty Acid Amide Hydrolase (FAAH) Inhibition Plays a Key Role in Counteracting Acute Lung Injury.International journal of molecular sciences · 2022Article
- The protective effect of natural medicines against excessive inflammation and oxidative stress in acute lung injury by regulating the Nrf2 signaling pathway.Frontiers in pharmacology · 2022Review
- Sirtuins and Sepsis: Cross Talk between Redox and Epigenetic Pathways.Antioxidants (Basel, Switzerland) · 2021Review
- Nitrosative Stress and Its Association with Cardiometabolic Disorders.Molecules (Basel, Switzerland) · 2020Review
- Modulation of Peroxynitrite Reduces Norepinephrine Requirements in Ovine MRSA Septic Shock.Shock (Augusta, Ga.) · 2019Article
- Peroxynitrite decomposition catalyst reduces vasopressin requirement in ovine MRSA sepsis.Intensive care medicine experimental · 2019Article
- Cardiovascular disease and resuscitated septic shock lead to the downregulation of the HIntensive care medicine experimental · 2017Article
- Time Response of Oxidative/Nitrosative Stress and Inflammation in LPS-Induced Endotoxaemia-A Comparative Study of Mice and Rats.International journal of molecular sciences · 2017Article
- OXIDATIVE STRESS IN A RAT MODEL OF COTTON SMOKE INHALATION-INDUCED PULMONARY INJURY.African journal of traditional, complementary, and alternative medicines : AJTCAM · 2016Article
- ExoU-induced redox imbalance and oxidative stress in airway epithelial cells during Pseudomonas aeruginosa pneumosepsis.Medical microbiology and immunology · 2015Article
- Hippocampal Neuroprotection by Minocycline and Epigallo-Catechin-3-Gallate Against Cardiopulmonary Bypass-Associated Injury.Brain pathology (Zurich, Switzerland) · 2015Article
- Diagnosis and management of inhalation injury: an updated review.Critical care (London, England) · 2015Review
- Strategies for Pharmacological Organoprotection during Extracorporeal Circulation Targeting Ischemia-Reperfusion Injury.Frontiers in pharmacology · 2015Review
- Differentiated control of deranged nitric oxide metabolism: a therapeutic option in sepsis?Critical care (London, England) · 2013Review
- Pneumonia-induced sepsis in mice: temporal study of inflammatory and cardiovascular parameters.International journal of experimental pathology · 2013Article
- Endothelial NOS (NOS3) impairs myocardial function in developing sepsis.Basic research in cardiology · 2013Article
- PARP-1 inhibitors DPQ and PJ-34 negatively modulate proinflammatory commitment of human glioblastoma cells.Neurochemical research · 2013Article
- Selective V(1a) agonism attenuates vascular dysfunction and fluid accumulation in ovine severe sepsis.American journal of physiology. Heart and circulatory physiology · 2012Article
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Authors and funding
12 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionDifferent isoforms of nitric oxide synthases (NOS) and determinants of oxidative/nitrosative stress play important roles in the pathophysiology of pulmonary dysfunction induced by acute lung injury (ALI) and sepsis. However, the time changes of these pathogenic factors are largely undetermined.
methodsTwenty-four chronically instrumented sheep were subjected to inhalation of 48 breaths of cotton smoke and instillation of live Pseudomonas aeruginosa into both lungs and were euthanized at 4, 8, 12, 18, and 24 hours post-injury. Additional sheep received sham injury and were euthanized after 24 hrs (control). All animals were mechanically ventilated and fluid resuscitated. Lung tissue was obtained at the respective time points for the measurement of neuronal, endothelial, and inducible NOS (nNOS, eNOS, iNOS) mRNA and their protein expression, calcium-dependent and -independent NOS activity, 3-nitrotyrosine (3-NT), and poly(ADP-ribose) (PAR) protein expression.
resultsThe injury induced severe pulmonary dysfunction as indicated by a progressive decline in oxygenation index and concomitant increase in pulmonary shunt fraction. These changes were associated with an early and transient increase in eNOS and an early and profound increase in iNOS expression, while expression of nNOS remained unchanged. Both 3-NT, a marker of protein nitration, and PAR, an indicator of DNA damage, increased early but only transiently.
conclusionsIdentification of the time course of the described pathogenetic factors provides important additional information on the pulmonary response to ALI and sepsis in the ovine model. This information may be crucial for future studies, especially when considering the timing of novel treatment strategies including selective inhibition of NOS isoforms, modulation of peroxynitrite, and PARP.
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