ArticleJournal of orthopaedic research : official publication of the Orthopaedic Research Society2011
Vimentin contributes to changes in chondrocyte stiffness in osteoarthritis.
Article in Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
44 citing papers in PubMed, 1 synthesis or guideline pooled it, 84 citations in OpenAlex.
- Alteration in cartilage matrix stiffness as an indicator and modulator of osteoarthritis.Bioscience reports · 2024Pooled it
- A deep neural network-enhanced multi-scale coupled model of patient-specific acoustic wave propagation for mechanotransduction stimulation of healthy and osteoarthritic knee articular cartilage.Biomechanics and modeling in mechanobiology · 2026Article
- Targeted Analysis of Chondrocyte Central Metabolites in Response to Cyclical Compression and Shear Deformations.Annals of biomedical engineering · 2026Article
- Targeting mechanotransduction components as therapeutic strategies in osteoarthritis.Molecular biology reports · 2026Review
- Viscoelastic recovery time of chondrocytes from monolayer and alginate cultures.bioRxiv : the preprint server for biology · 2026Article
- Metabolomic Profiling and Characterization of a Novel 3D Culture System for Studying Chondrocyte Mechanotransduction.Cellular and molecular bioengineering · 2025Article
- Mechanical cues enhance chondrocyte function: Insights from mechanoreception, regulation, and biological responses.Mechanobiology in medicine · 2025Review
- Computational prediction of cellular elastic modulus from mechanosensitive gene expression at multiple biological levels.BioData mining · 2025Article
- Osmolarity-Induced Altered Intracellular Molecular Crowding Drives Osteoarthritis Pathology.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Article
- Molecular determinants of intrinsic cellular stiffness in health and disease.Biophysical reviews · 2022Review
- Intra-articular injection of flavopiridol-loaded microparticles for treatment of post-traumatic osteoarthritis.Acta biomaterialia · 2022Article
- Proteomic Analyses Reveals the Mechanism of Acupotomy Intervention on the Treatment of Knee Osteoarthritis in Rabbits.Evidence-based complementary and alternative medicine : eCAM · 2022Article
- Nuclear envelope mechanobiology: linking the nuclear structure and function.Nucleus (Austin, Tex.) · 2021Review
- Articular Chondrocyte Phenotype Regulation through the Cytoskeleton and the Signaling Processes That Originate from or Converge on the Cytoskeleton: Towards a Novel Understanding of the Intersection between Actin Dynamics and Chondrogenic Function.International journal of molecular sciences · 2021Review
- Mechanotransduction and Stiffness-Sensing: Mechanisms and Opportunities to Control Multiple Molecular Aspects of Cell Phenotype as a Design Cornerstone of Cell-Instructive Biomaterials for Articular Cartilage Repair.International journal of molecular sciences · 2020Review
- Qualitative analysis of contribution of intracellular skeletal changes to cellular elasticity.Cellular and molecular life sciences : CMLS · 2020Review
- Long noncoding RNA LINC00461 induced osteoarthritis progression by inhibiting miR-30a-5p.Aging · 2020Article
- VAMP3 and SNAP23 as Potential Targets for Preventing the Disturbed Flow-Accelerated Thrombus Formation.Frontiers in cell and developmental biology · 2020Article
- Primary human chondrocytes respond to compression with phosphoproteomic signatures that include microtubule activation.Journal of biomechanics · 2019Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Actin and tubulin cytoskeletal components are studied extensively in chondrocytes, but less is known about vimentin intermediate filaments. In other cell types, vimentin is a determinant of cell stiffness and disruption of vimentin networks weakens the mechanical integrity of cells. Changes in vimentin organization correlate with osteoarthritis progression, but the functional consequences of these changes remain undetermined in chondrocytes. The objective of this study was to compare the contribution of vimentin to the mechanical stiffness of primary human chondrocytes isolated from normal versus osteoarthritic cartilage. Chondrocytes were embedded in alginate and vimentin networks disrupted with acrylamide. Constructs were imaged while subjected to 20% nominal strain on a confocal microscope stage, and the aspect ratios of approximately 1,900 cells were measured. Cytosolic stiffness was estimated with a finite element model, and live-cell imaging of GFP-vimentin was used to further analyze the nature of vimentin disruption. Vimentin in normal chondrocytes formed an inner cage-like network that was substantially stiffer than the rest of the cytosol and contributed significantly to overall cellular stiffness. Disruption of vimentin reduced stiffness approximately 2.8-fold in normal chondrocytes. In contrast, osteoarthritic chondrocytes were less stiff and less affected by vimentin disruption. This 3D experimental system revealed contributions of vimentin to chondrocyte stiffness previously not apparent, and correlated changes in vimentin-based chondrocyte stiffness with osteoarthritis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.