ReviewMolecular bioSystems2010
Multicolor single-molecule FRET to explore protein folding and binding.
Review in Molecular bioSystems, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed.
- Uninterrupted optical resolution of identical point scatterers undergoing nanometric changes in distance.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Self-assembled [2 + 3] organic-imine cage as an artificial light harvester for the photocatalytic organic transformation in an aqueous medium.RSC advances · 2025Article
- Three-color single-molecule maximum likelihood analysis of folding and binding of diffusing molecules.Biophysical journal · 2025Article
- Unravelling molecular dynamics in living cells: Fluorescent protein biosensors for cell biology.Journal of microscopy · 2025Review
- Technologies for investigating single-molecule chemical reactions.National science review · 2024Review
- Energy landscape of conformational changes for a single unmodified protein.Npj biosensing · 2024Article
- Structural Ensemble of the Insulin Monomer.Biochemistry · 2021Article
- Single-molecule FRET and conformational analysis of beta-arrestin-1 through genetic code expansion and a Se-click reaction.Chemical science · 2021Article
- Sub-20 nm Core-Shell-Shell Nanoparticles for Bright Upconversion and Enhanced Förster Resonant Energy Transfer.Journal of the American Chemical Society · 2019Article
- Single-molecule fluorescence studies of intrinsically disordered proteins and liquid phase separation.Biochimica et biophysica acta. Proteins and proteomics · 2019Review
- A Bayesian Nonparametric Approach to Single Molecule Förster Resonance Energy Transfer.The journal of physical chemistry. B · 2019Article
- Three-Color Single-Molecule FRET and Fluorescence Lifetime Analysis of Fast Protein Folding.The journal of physical chemistry. B · 2018Article
- Site-Specific Three-Color Labeling of α-Synuclein via Conjugation to Uniquely Reactive Cysteines during Assembly by Native Chemical Ligation.Cell chemical biology · 2018Article
- Transition Path Times Measured by Single-Molecule Spectroscopy.Journal of molecular biology · 2018Review
- Toward dynamic structural biology: Two decades of single-molecule Förster resonance energy transfer.Science (New York, N.Y.) · 2018Review
- Accurate Transfer Efficiencies, Distance Distributions, and Ensembles of Unfolded and Intrinsically Disordered Proteins From Single-Molecule FRET.Methods in enzymology · 2018Article
- Analysis of Fluorescence Lifetime and Energy Transfer Efficiency in Single-Molecule Photon Trajectories of Fast-Folding Proteins.The journal of physical chemistry. B · 2016Article
- Deciphering Complexity in Molecular Biophysics with Single-Molecule Resolution.Journal of molecular biology · 2016Review
- Quantitative interpretation of FRET experiments via molecular simulation: force field and validation.Biophysical journal · 2015Article
- Probing protein disorder and complexity at single-molecule resolution.Seminars in cell & developmental biology · 2015Review
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Proper protein function in cells, tissues and organisms depends critically on correct protein folding or interaction with partners. Over the last decade, single-molecule FRET (smFRET) has emerged as a powerful tool to probe complex distributions, dynamics, pathways and landscapes in protein folding and binding reactions, leveraging its ability to avoid averaging over an ensemble of molecules. While smFRET was practiced in a two-color form until recently, the last few years have seen the development of enhanced multicolor smFRET methods that provide additional structural information permitting us to probe more complex mechanisms. In this review, we provide a brief introduction to the smFRET technique, then follow with advanced multicolor measurements and end with ongoing methodology developments in microfluidics and protein labeling that are beginning to make these techniques more broadly applicable to answering a number of key questions about folding and binding.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.